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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海信裕生物科技有限公司
- 库存:
50
- 克隆性:
多克隆
- 保质期:
1年
- 保存条件:
常温
- 规格:
100μL
PRODUCT TYPE: Secondary Antibody
PHYSICAL STATE: Liquid
ANTIBODY SOURCE: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
ANTIBODY PURITY: The antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins.
SUGGESTED DILUTION RANGE
1:500 - 1:5000 for immunohisto/cytochemistry1:5000 - 1:100000 for ELISA and Western blotting with chromogenic substrates
1:10000 - 1:200000 for Western blotting with ECL substrates. STORAGE BUFFER: 0.01M Sodium phosphate, 0.25M NaCl, 50% Glycerol, pH7.6
STABILIZER: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
PRESERVATIVE: None (Warining: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
STORAGE INSTRUCTIONS: Store at -20°C. Avoid repeated freeze / thaw cycles.
NOTE: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Nothing
disclosed herein is to be construed as a recommendation to use this product in violation of any patents. The information presented above is believed to be accurate. However, said information and product are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. We cannot be responsible for patent infringements or other violations that may occur with the use of this product风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人抗风疹病毒IgG抗体(anti-RV IgG)酶联免疫分析(ELISA)
中依次加入抗风疹病毒 IgG 抗体( anti-RV IgG ),再与 HRP 标记的抗原结合,形成抗原 - 抗体 - 酶标抗原复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体( anti-RV IgG )呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人抗风疹病毒 IgG 抗体( anti-RV IgG )浓度。 试剂盒组成
Anti-Factor H Autoantibodies Assay
of autoantibodies directed against Factor H (FH), the main plasmatic alternative complement pathway regulatory protein. Their presence induces an acquired functional FH deficiency. The anti-FH autoantibodies screening must be performed at the very onset
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
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