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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海信裕生物科技有限公司
- 库存:
50
- 克隆性:
多克隆
- 保质期:
1年
- 保存条件:
常温
- 规格:
100μL
Catalog No. PH0650
Product Type Secondary Antibody
Name HRP AffiniPure Goat Anti-Rabbit IgG (H+L)
Size 100ul
Storage Store at -20℃ Avoid repeated freeze / thaw cycles
Physical State Liquid
Antibody Source The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
Antibody Purity The antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins.
Application
1:500 - 1:5000 for immunohisto/cytochemistry
1:5000 - 1:100000 for ELISA and Western blotting with chromogenic substrates
1:10000 - 1:200000 for Western blotting with ECL substrates.
Storage Buffer 0.01M Sodium phosphate, 0.25M NaCl, 50% Glycerol, pH7.6
Stabilizer 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative None (Warining: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Note The product listed herein is for research use only and is not intended for use in human or clinical diagnosis.
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文献和实验人抗风疹病毒IgG抗体(anti-RV IgG)酶联免疫分析(ELISA)
中依次加入抗风疹病毒 IgG 抗体( anti-RV IgG ),再与 HRP 标记的抗原结合,形成抗原 - 抗体 - 酶标抗原复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体( anti-RV IgG )呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人抗风疹病毒 IgG 抗体( anti-RV IgG )浓度。 试剂盒组成
Anti-Factor H Autoantibodies Assay
of autoantibodies directed against Factor H (FH), the main plasmatic alternative complement pathway regulatory protein. Their presence induces an acquired functional FH deficiency. The anti-FH autoantibodies screening must be performed at the very onset
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
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