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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
nickel ions, giving a highly selective interaction that allows purification of tagged proteins or protein complexes from 95% homogeneity in just one step (1 ,2 ). The tight association between the tag and the Ni-NTA resin allows contaminants
6xffis-Ni-NTA Chromatography as a Superior Technique in Recombinant Protein Expression/Purification
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
the CRC filter (35-µm) into the CRC. Place the CRC in a standard microcentrifuge tube. Add ~30 µL of the Ni-NTA resin slurry to the bottom of the CRC tube. Drain the column by centrifugation at 120g for 1 min at room temperature. These centrifugation
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