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- MedChemExpress(MCE)
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- 2025年12月05日
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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期：询盘
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MedChemExpress LLC
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TAMRA-probe 1
MCE 国际站：TAMRA-probe 1
产品活性：TAMRA-probe 1 是一种常用的用于标记的荧光探针。
研究领域：Others
作用靶点：Fluorescent Dye
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文献和实验by ethidium bromide staining and UV illumination (see note 3). Stop transcription reaction by addition of 5μl SDS, 2μl 2-mercaptoethanol and incubation at 80°C for 5 minutes. Store probe at -20°C until required. Multiple rounds of thawing / freezing
Prehybridization and probe labeling
1 mL TOTAL 200 mL Prehybridize membranes for at least 6 hours to overnight. Probe labeling Heat water to boiling
Transcription of cRNA probe Reagents/Solutions DNA (PCR or plasmid) with bacteriophage RNA polymerase promoter in suitable orientation (see note 1 ) ~1µg/µl in TE buffer 10mM MgCl2 5x Transcription Buffer 0.2
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