TaqMan TAMRA PROBE 6,000 PMOLE

实时荧光Taqman 探针设计的几个要点

信标Molecular Beacon。 广泛使用的TaqMan探针法是指PCR扩增时在加入一对引物 的同时另外加入一个特异性的荧光探针，该探针只与模板特异性地结合，其结合位点在两条引物之间。探针的5′端标记有荧光报告基团(Reporter, R)，如FAM、VIC等，3′端标记有荧光淬灭基团(Quencher, Q)，如TAMRA等。当探针完整的时候，5′端报告基团经仪器光源激发的荧光正好被近距离的3′端荧光基团淬灭，仪器检测不到5′端报告基团所激发的荧光信号(就是说5’荧光基团的发射波长正好

CNV Analysis Using TaqMan Copy Number Assays

. One TaqMan TAMRA probe for detecting the reference sequence, including VIC reporter dye attached to the 5′ end and TAMRA quencher attached to the 3′ end

Quantitation of Liver-Stage Parasites by Automated TaqMan Real-Time PCR

. However, the measurement of bloodstage parasitemia is a slow, painstaking process requiring a high level of technical ability. In addition, a biological amplification of parasites occurs in hepatocytes, with one infected hepatocyte yielding approx 4000 -0,000 merozoites