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2500次/200次/500次
规格: | 2500次 | 产品价格: | ¥1600.0 |
---|---|---|---|
规格: | 200次 | 产品价格: | ¥180.0 |
规格: | 500次 | 产品价格: | ¥350.0 |
微板(microplate)测定方案 | 标准比色杯测定方案 | ||||
标号 | 蛋白浓度(µg/ml) | 标准或待测蛋白体积 (µl) | WR工作试剂(µl) | 标准或待测蛋白体积 (ml) | WR工作试剂(ml) |
1 | 0 | 50 | 200 | 0.2 | 0.8 |
2 | 1.25 | 50 | 200 | 0.2 | 0.8 |
3 | 2.5 | 50 | 200 | 0.2 | 0.8 |
4 | 5 | 50 | 200 | 0.2 | 0.8 |
5 | 10 | 50 | 200 | 0.2 | 0.8 |
6 | 20 | 50 | 200 | 0.2 | 0.8 |
7 | 40 | 50 | 200 | 0.2 | 0.8 |
8 | 80 | 50 | 200 | 0.2 | 0.8 |
待测样品 | 50 | 200 | 0.2 | 0.8 |
Buffer Systems | Sodium phosphate 25 mM |
Bicine, pH 8.4 20 mM | Sucrose 40% |
Bis-Tris, pH 6.5 33 mM | Sodium ortho-Vanadate in PBS, pH 7.2, 1 mM |
Calcium chloride in TBS, pH 7.2 10 mM | Urea 3 M |
CHES, pH 9.0 100 mM | Chelating agents |
Cobalt chloride in TBS, pH 7.2 0.8 M | EDTA 10 mM |
Ferric chloride in TBS, pH 7.2 10 mM | EGTA,any level, not compatible |
HEPES 100 mM | Sodium citrate 200 mM |
MOPS, pH 7.2 100 mM | Detergents |
Nickel chloride in TBS 10 mM | Brij-35 5% |
PBS; no interference | Brij-52 1% |
NaCl (0.15 M), pH 7.2, no interference | CHAPS 5% |
PIPES, pH 6.8 100 mM | CHAPSO 5% |
Sodium acetate, pH 4.8 200 mM | Deoxycholic acid 5% |
Sodium citrate, pH 4.8 or pH 6.4 200 mM | Nonidet P-40 (Igepal CA-630) 5% |
Tricine, pH 8.0 25 mM | SDS 5% |
Triethanolamine, pH 7.8 25 mM | Span 20 1% |
Tris 250 mM | Triton X-100 5% |
TBS buffer, no interference | Triton X-114 1% |
1 x SDS-PAGE loading buffer, no interference | Tween-20 5% |
Zinc chloride (10 mM) in TBS, pH 7.2, 10 mM | Tween-60 5% |
Buffer Additives | Tween-80 5% |
Ammonium sulfate 1.5 mM | Zwittergents 1% |
Aprotinin 10 mg/L | Reducing & Thiol Containing Agents |
Glucose 10 mM | Dithioerythritol (DTE) 1 mM |
Glycerol 10% | Dithiothreitol (DTT) 1 mM |
Guanidine•HCl 4 M | 2-Mercaptoethanol 1 mM |
HCl 100 mM | Tributyl Phosphine 0.01% |
Imidazole 50 mM | Solvents |
Leupeptin 10 mg/L | |
PMSF 1 mM | Acetonitrile 10% |
Sodium azide 0.20% | DMF 10% |
Sodium bicarbonate 100 mM | DMSO 10% |
Sodium chloride 1 M | Ethanol 10% |
Sodium hydroxide 100 mM | Methanol 10% |
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