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- 碧云天（beyotime）
- P0011
- 2025年12月18日
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| 规格： | 200次 | 产品价格： | ￥172.0 |
|---|---|---|---|
| 规格： | 500次 | 产品价格： | ￥329.0 |
| 规格： | 5000次 | 产品价格： | ￥1592.0 |
BCA蛋白浓度测定试剂盒(BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方法之一BCA法研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。
灵敏度高，检测浓度下限达到25μg/ml，最小检测蛋白量达到0.5μg，待测样品体积为1-20μl。
在50-2000μg/ml浓度范围内有较好的线性关系。
BCA法测定蛋白浓度不受绝大部分样品中的化学物质的影响，可以兼容样品中高达5%的SDS，5%的Triton X-100，5%的Tween 20、60、80。但本试剂盒受螯合剂和略高浓度的还原剂的影响，需确保EDTA低于10mM，无EGTA，二硫苏糖醇(DTT)低于1mM，β-巯基乙醇(β-Mercaptoethanol)低于0.01%。不适用BCA法时建议试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
每个试剂盒可以检测5000个样品。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| P0011-1 | BCA试剂 A | 500ml×2 |
| P0011-2 | BCA试剂 B | 30ml |
| P0011-3 | 蛋白标准(BSA) | 30mg×2 |
| P0011-4 | 蛋白标准配制液 | 5ml |
| ― | 说明书 | 1份 |
保存条件：
室温保存。蛋白标准配制成溶液后-20℃冻存。
注意事项：
需酶标仪一台，测定波长为540-595nm之间，562nm最佳。需96孔板。如果没有酶标仪，也可以使用普通的分光光度计测定，但测定时，需根据比色皿的最小检测体积，适当加大BCA工作液的用量使不小于最小检测体积，样品和标准品的用量可相应按比例放大也可不变。使用分光光度计测定蛋白浓度时，每个试剂盒可以测定的样品数量可能会显著减少。
如发现样品稀释液或裂解液本身就有较高背景，请试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
为了加快BCA法测定蛋白浓度的速度可以适当用微波炉加热，但是切勿过热。
EDTA浓度必须小于10mM，不兼容EGTA。不适用BCA法时，请试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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文献和实验该产品被引用文献
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Studies on PEG-modified SLNs loading vinorelbine bitartrate (I): preparation and evaluation in vitro.
INT J PHARMACOL. 2008 Jul 9;359(1-2):104-10. (IF 0.692)
76. An R, Dong C, Lei Y, Han L, Li P, Chen J, Wang G, Shi Q, Gao C, Jiang H, Zhou W, Han J, Chu Y, Dong X.
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PrP mutants with different numbers of octarepeat sequences are more susceptible to the oxidative stress.
SCI CHINA LIFE SCI. 2008 Jul;51(7):630-9. (IF 4.611)
77. Wei Y, Weng D, Li F, Zou X, Young DO, Ji J, Shen P.
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Involvement of JNK regulation in oxidative stress-mediated murine liver injury by microcystin-LR.
Apoptosis. 2008 Aug;13(8):1031-42. (IF 4.543)
78. Tian SL, Wang XY, Ding GH.
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Repeated electro-acupuncture attenuates chronic visceral hypersensitivity and spinal cord NMDA receptor phosphorylation in a rat irritable bowel syndrome model.
Life Sci. 2008 Aug 29;83(9-10):356-63. (IF 3.647)
79. Chen M, Li Y, Yang T, Wang Y, Bai Y, Xie X.
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ADMA induces monocyte adhesion via activation of chemokine receptors in cultured THP-1 cells.
Cytokine. 2008 Aug;43(2):149-59. (IF 2.952)
80. Hu X, Zhou J, Zhang N, Tan H, Gao C.
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Preparation and properties of an injectable scaffold of poly(lactic-co-glycolic acid) microparticles/chitosan hydrogel.
J MECH BEHAV BIOMED. 2008 Oct;1(4):352-9. (IF 3.372)
81. Sun J, Wu Y, Wang J, Ma F, Liu X, Li Q.
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Novel translationally controlled tumor protein homologue in the buccal gland secretion of Lampetra japonica.
Biochimie. 2008 Nov-Dec;90(11-12):1760-8. (IF 3.413)
82. Zhu M, Gu F, Shi J, Hu J, Hu Y, Zhao Z.
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Increased oxidative stress and astrogliosis responses in conditional double-knockout mice of Alzheimer-like presenilin-1 and presenilin-2.
FREE RADICAL BIO MED. 2008 Nov 15;45(10):1493-9. (IF 6.17)
83. Liu XJ, Wang WC, Wang HJ, Zhang LL, Liu LQ, Wang YJ, Wang CB.
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A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway.
Chinese Journal of Oceanology and Limnology. 2009;27(3):587-593. (IF 0.741)
84. Fan L, Lin C, Zhuo S, Chen L, Liu N, Luo Y, Fang J, Huang Z, Lin Y, Chen J.
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Transplantation with survivin-engineered mesenchymal stem cells results in better prognosis in a rat model of myocardial infarction.
Eur J Heart Fail. 2009;11(11):1023-30. (IF 11.627)
85. Yi Q, Wen X, Li L, He B, Nie Y, Yao Wu Y, Zhang Z, Gu Z.
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The chiral effects on the responses of osteoblastic cells to the polymeric substrates.
European Polymer Journal. 2009;45:1970–8. (IF 3.621)
86. ZHANG ZY, LIU XJ, LIU T, YAN L, WANG YJ, WANG CB.
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Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90.
Chinese Journal of Oceanology and Limnology. 2009;27(3):594-599. (IF 0.741)
87. WU T, NAN KH, CHEN JD, JIN D, JIANG S, ZHAO PR, XU JC, DU H, ZHANG XQ, LI JW, PEI GX.
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A new bone repair scaffold combined with chitosan/hydroxyapatite and sustained releasing icariin.
CHINESE SCI BULL. 2009;54(17):2953-2961. (IF 1.649)
88. Li W, Liu Y, Zhang JW, Ai CZ, Xiang N, Liu HX, Yang L.
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Anti-androgen-independent prostate cancer effects of ginsenoside metabolites in vitro: mechanism and possible structure-activity relationship investigation.
Arch Pharm Res. 2009 Jan;32(1):49-57. (IF 2.934)
89. Yuan Q, Li PD, Li BH, Yang XZ, Xu SB, Liu XH, Zhou FX, Zhang WJ.
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Differential IL-4/Stat6 activities correlate with differential expression of regulatory genes SOCS-1, SHP-1, and PP2A in colon cancer cells.
J CANCER RES CLIN. 2009 Jan;135(1):131-140. (IF 3.656)
90. Wang YL, Wang CY, Zhang BJ, Zhang ZZ.
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Shenfu injection suppresses apoptosis by regulation of Bcl-2 and caspase-3 during hypoxia/reoxygenation in neonatal rat cardiomyocytes in vitro.
Mol Biol Rep. 2009 Feb;36(2):365-70. (IF 1.402)
91. Liu XS, Jiang J, Jiao XY, Wu YE, Lin JH, Cai YM.
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Lycorine induces apoptosis and down-regulation of Mcl-1 in human leukemia cells.
Cancer Lett. 2009 Feb 8;274(1):16-24. (IF 7.36)
92. Gao R, Wang J, Shen Y, Lei M, Wang Z.
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Functional expression of voltage-gated sodium channels Nav1.5 in human breast caner cell line MDA-MB-231.
J HUAZHONG U SCI-MED. 2009 Feb;29(1):64-7. (IF 1.151)
93. Liu X, Zhang Z, Li P, Zhu L, Wang Y, Wang C.
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Polypeptide from Chlamys farreri modulates UVB-induced activation of NF-kappaB signaling pathway and protection HaCaT cells from apoptosis.
REGUL PEPTIDES. 2009 Feb 25;153(1-3):49-55. (IF 1.813)
94. Jin D, Ojcius DM, Sun D, Dong H, Luo Y, Mao Y, Yan J.
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Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways.
Infect Immun. 2009 Feb;77(2):799-809. (IF 3.201)
95. Kang Y, Hu M, Zhu Y, Gao X, Wang MW.
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Antioxidative effect of the herbal remedy Qin Huo Yi Hao and its active component tetramethylpyrazine on high glucose-treated endothelial cells.
Life Sci. 2009 Mar 27;84(13-14):428-36. (IF 3.647)
96. Xu J, Wang S, Lin Y, Cao L, Wang R, Chi Z.
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Ghrelin protects against cell death of hippocampal neurons in pilocarpine-induced seizures in rats.
Neurosci Lett. 2009 Mar 27;453(1):58-61. (IF 2.274)
97. Jia H, Qi X, Fang S, Jin Y, Han X, Wang Y, Wang A, Zhou H.
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Carnosine inhibits high glucose-induced mesangial cell proliferation through mediating cell cycle progression.
REGUL PEPTIDES. 2009 Apr 10;154(1-3):69-76. (IF 1.813)
98. Zhang LF, Peng SQ, Wang S.
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Decreased aortic contractile reaction to 5-hydroxytryptamine in rats with long-term hypertension induced by lead (Pb(2+)) exposure.
Toxicol Lett. 2009 Apr 25;186(2):78-83. (IF 3.569)
99. Dong C, Wang Y, Zhu YZ.
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Asymmetric synthesis and biological evaluation of Danshensu derivatives as anti-myocardial ischemia drug candidates.
BIOORG MED CHEM LETT. 2009 May 1;17(9):3499-507. (IF 2.572)
100. Xiao X, Liu A, Wen H, Tian Y, Ni J, Liu G.
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Expression and localization of transcription factor Ets-1 in the rat ovary during the estrous cycle and pregnancy.
Fertil Steril. 2009 May;91(5):1998-2005. (IF 6.312)
101. Cheng B, Song J, Zou Y, Wang Q, Lei Y, Zhu C, Hu C.
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Responses of vascular smooth muscle cells to estrogen are dependent on balance between ERK and p38 MAPK pathway activities.
Int J Cardiol. 2009 May 29;134(3):356-65. (IF 3.229)
102. Chen MF, Li YJ, Yang TL, Lou B, Xie XM.
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Losartan inhibits monocytic adhesion induced by ADMA via downregulation of chemokine receptors in monocytes.
Eur J Clin Pharmacol. 2009 May;65(5):457-64. (IF 2.641)
103. Zhang DL, Chen YQ, Jiang X, Ji TT, Mei B.
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Oxidative damage increased in presenilin1/presenilin2 conditional double knockout mice.
Neurosci Bull. 2009 Jun;25(3):131-7. (IF 4.326)
104. Wang XD, Meng MX, Gao LB, Liu T, Xu Q, Zeng S.
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Permeation of astilbin and taxifolin in Caco-2 cell and their effects on the P-gp.
INT J PHARMACOL. 2009 Aug 13;378(1-2):1-8. (IF 0.692)
105. Chen B, Gao XQ, Yang CX, Tan SK, Sun ZL, Yan NH, Pang YG, Yuan M, Chen GJ, Xu GT, Zhang K, Yuan QL.
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Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral ischemia in rats.
Brain Res. 2009 Aug 11;1284:1-11. (IF 2.733)
106. Ding D, Huang P, Sun H, Xue Q, Pan Y, Chen S.
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Identification of protein components and quantitative immunoassay for SEC2 in staphylococcin injection.
J PHARMACEUT BIOMED. 2009 Aug 15;50(1):79-85. (IF 3.209)
107. Liu X, Shi S, Ye J, Liu L, Sun M, Wang C.
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Effect of polypeptide from Chlamys farreri on UVB-induced ROS/NF-kappaB/COX-2 activation and apoptosis in HaCaT cells.
J PHOTOCH PHOTOBIO B. 2009 Aug 3;96(2):109-16. (IF 4.383)
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Overexpression of the Interferon regulatory factor 4-binding protein in human colorectal cancer and its clinical significance.
Cancer Epidemiol. 2009 Aug;33(2):130-6. (IF 2.179)
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Structure-transfection activity relationships with glucocorticoid-polyethyl-enimine conjugate nuclear gene delivery systems.
Biomaterials. 2009 Aug;30(22):3780-9. (IF 10.317)
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Radiation enhances suicide gene therapy in radioresistant laryngeal squamous cell carcinoma via activation of a tumor-specific promoter.
Cancer Lett. 2009 Sep 28;283(1):20-8. (IF 7.36)
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Cell Biol Int. 2009 Sep;33(9):918-25. (IF 2.571)
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Inhibition of farnesylpyrophosphate synthase prevents angiotensin II-induced hypertrophic responses in rat neonatal cardiomyocytes: involvement of the RhoA/Rho kinase pathway.
FEBS Lett. 2009 Sep 17;583(18):2997-3003. (IF 3.057)
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H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and differentiation.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Sep;108(3):368-75. (IF 0.27)
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FEBS Lett. 2009 Sep 3;583(17):2811-8. (IF 3.057)
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A novel marine compound xyloketal B protects against oxidized LDL-induced cell injury in vitro.
Biochem Pharmacol. 2009 Oct 15;78(8):941-50. (IF 4.96)
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BIOCHEM BIOPH RES CO. 2009 Oct 23;388(3):595-600. (IF 2.985)
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Proteome analysis of a single zebrafish embryo using three different digestion strategies coupled with liquid chromatography-tandem mass spectrometry.
Anal Biochem. 2009 Nov 15;394(2):177-85. (IF 2.877)
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Cancer Lett. 2009 Nov 1;284(2):229-37. (IF 7.36)
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Behav Brain Res. 2009 Dec 14;205(1):32-7. (IF 2.977)
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Nuclear proteome analysis of cisplatin-treated HeLa cells.
MUTAT RES-REV MUTAT. 2010;691(1-2):1-8. (IF 5.803)
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Food Control. 21 (2010) 89–96. (IF 4.258)
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PHARMACOL BIOCHEM BE. 2010;96(3):301-5. (IF 2.519)
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Arch Biochem Biophys. 2010 15;496(2):77-83. (IF 3.391)
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Acta Histochem. 2010;112(3):215-21. (IF 2.107)
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CHEM-BIOL INTERACT. 2010;183(1):142-53. (IF 3.723)
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Mechanistic understanding of the different effects of Wuzhi Tablet (Schisandra sphenanthera extract) on the absorption and first-pass intestinal and hepatic metabolism of Tacrolimus (FK506).
INT J PHARMACOL. 2010;389(1-2):114-21. (IF 0.692)
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Neurosci Lett. 2010;474(1):22-5. (IF 2.274)
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Exp Gerontol. 2010;45(11):842-7. (IF 3.376)
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Gene structure, recombinant expression and functional characterization of grass carp leptin.
GEN COMP ENDOCR. 2010;166(1):117-27. (IF 2.426)
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Neferine, a bisbenzylisoquinline alkaloid attenuates bleomycin-induced pulmonary fibrosis.
Eur J Pharmacol. 2010;627(1-3):304-12. (IF 3.263)
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Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution treated nanostructural titanium surface.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110(4):e13-22. (IF 0.27)
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