- 询价
- 近岸蛋白(Novoprotein)
- DRA35
- 2026年07月10日
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- 文献和实验
- 技术资料
- 英文名：
Recombinant 2019-nCoV S1 Protein
- 保存条件：
Reconstituted protein solution should be stored at ≤ -20°C.
- 规格：
50μg/500μg/1mg
| 规格： | 50μg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 500μg | 产品价格： | 询价 |
| 规格： | 1mg | 产品价格： | 询价 |
Recombinant 2019-nCoV S1 Protein
Catalog #: DRA35 Derived From: Human Cells
Description:
Recombinant 2019-nCoV S1 Protein is produced by our Mammalian expression system and the target gene encoding Gln14-Arg685 is expressed.
Accession: QHD43416.1
Names(Known as): S1 protein; 2019-nCoV S1 protein; coronavirus S1 Protein; cov S1 Protein
Quality Control:
Mol Mass: 78.3kDa AP Mol Mass: 100-130kDa, reducing conditions
Purity: Greater than 85% as determined by reducing SDS-PAGE.
Bioactivity:
Immobilized Human ACE-2-Fc(Cat#C05Y) at 10μg/ml (100 μl/well) can bind 2019-nCoV S1 Protein* (Cat#DRA35).*: Biotinylated by NHS-biotin prior to testing. The ED50 of 2019-nCoV S1 Protein* (Cat#DRA35)is 1.18 ug/ml.
Formulation:
Supplied as a 0.2 μM filtered solution of PBS, pH 7.4
Shipping:
The product is shipped on dry ice pack.
Upon receipt, store it immediately at the temperature listed below.
Storage:
Reconstituted protein solution should be stored at ≤ -20°C.
Purification:
Affinity purification chromatography.
Immunoreactivity:
N.A.
Application:
Immunogen, calibrator or standard.
Background:
Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY
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文献和实验Protein Extraction From Mammalian Tissues
For Western blotting and coimmunoprecipitation (coIP), protein samples must be extracted from tissues. The protocol described in this chapter has been used to extract clock proteins from mammalian tissues as diverse as liver, kidney
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag（Cleavage Under Targets and Tagmentation）是蛋白-DNA 互作的一大革新技术，它不需要使用甲醛交联以及免疫共沉淀，而是通过针对靶蛋白（如转录因子、染色质重塑蛋白）的抗体和 Protein A/G 的介导，使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头，经 PCR 扩增后即可形成用于高通量测序的文库（见图 1 ）。CUT &Tag 技术具有细胞投入量低、信噪
Non-fluorescent protein/RNA double-labeling
1. Collect embryos, dechorionate and fix in a (1:1) mixture of heptane:fix. Fix is 5% formaldehyde, 0.05M EGTA in 1X PBS. pH 7.0. Devitellinize embryos by shaking in an equal mixture of methanol:heptane (embryos will sink
