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- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
PerCP/Cyanine5.5 anti-human CD4
- 供应商：
上海善然生物科技有限公司
- 保存条件：
低温
- 规格：
25 tests
PerCP/Cyanine5.5 anti-human CD4 Antibody
Catalog# / Size 317427 / 25 tests
317428 / 100 tests
Clone OKT4
Workshop HCDM listed
Other Names T4
Isotype Mouse IgG2b, κ
Description CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed
on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the
Ig superfamily, recognizes antigens associated with MHC class II molecules and participates
in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary
receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Product Details
Reactivity Human, Cross-Reactivity: Chimpanzee, Cynomolgus, Rhesus
Antibody Type Monoclonal
Host Species Mouse
Immunogen Human peripheral T cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin
USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under
optimal conditions.
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup
tool if you have a lot number.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric
analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100
µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser Blue Laser (488 nm)
Application Notes The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional
reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and
blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed
paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free,
0.2 μm filtered) is recommended for functional assays (Cat. No. 317404).
In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.
Additional Product Notes BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye
molecule remains the same, so you should expect the same quality and performance from our
PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
(PubMed link indicates
BioLegend citation)
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
Product Citations
Version: 5 Revision Date: 12/05/2018
1. Shi Y, et al. 2014. Proc Natl Acad Sci U S A. 111:3092.. PubMed
2. Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
3. Kongsbak M, et al. 2014. PLoS One. 9:96695. PubMed
4. Jin J, et al. 2014. PLoS One. 9:104753. PubMed
5. Meira M, et al. 2014. Mult Scler. 20:1363. PubMed
6. Florek N, et al. 2014. J Virol. 88:13418. PubMed
7. Crompton J, et al. 2015. Cancer Res. 75:296. PubMed
8. Ma G, et al. 2015. Proc Natl Acad Sci U S A. 112:2216. PubMed
9. Ishigaki K, et al. 2015. Sci Rep. 5: 12937. PubMed
10. Bego M, et al. 2015. J Vis Exp. 103: 51207. PubMed
11. Heery C, et al. 2015. Cancer Immunol Res. 3: 1248 - 1256. PubMed
12. Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed
RRID AB_1186124 (BioLegend Cat. No. 317427)
AB_1186122 (BioLegend Cat. No. 317428)
Antigen Details
Structure Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution T cell subset, majority of thymocytes, monocytes/macrophages
Function MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor MHC class II molecules, HIV gp120, IL-16
Cell Type Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area Immunology
Molecular Family CD Molecules
Antigen References 1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID 920
Related Protocols
Cell Surface Flow Cytometry Staining Protocol
Brilliant Violet 650™ anti-human CD4, Purified anti-human CD4, LEAF™ Purified anti-human CD4, Biotin anti-human CD4, FITC antihuman
CD4, PE anti-human CD4, PE/Cy5 anti-human CD4, PE/Cy7 anti-human CD4, APC anti-human CD4, APC/Cyanine7 anti-human
CD4, Alexa Fluor® 488 anti-human CD4, Alexa Fluor® 647 anti-human CD4, Alexa Fluor® 700 anti-human CD4, Pacific Blue™ antihuman
CD4, PerCP anti-human CD4, Brilliant Violet 421™ anti-human CD4, Brilliant Violet 605™ anti-human CD4, Brilliant Violet
711™ anti-human CD4, Brilliant Violet 785™ anti-human CD4, Brilliant Violet 510™ anti-human CD4, Brilliant Violet 570™ anti-human
CD4, PE/Dazzle™ 594 anti-human CD4, TotalSeq™-A0922 anti-human CD4
Other Formats
Product Data
Human peripheral blood lymphocytes
were stained with CD4 (clone OKT4)
PerCP/Cyanine5.5 (filled histogram) or
Mouse IgG2b, κ PerCP/Cyanine5.5
isotype control (open histogram).
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written
approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
commercial use.
BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
Catalog# / Size 317427 / 25 tests
317428 / 100 tests
Clone OKT4
Workshop HCDM listed
Other Names T4
Isotype Mouse IgG2b, κ
Description CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed
on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the
Ig superfamily, recognizes antigens associated with MHC class II molecules and participates
in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary
receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Product Details
Reactivity Human, Cross-Reactivity: Chimpanzee, Cynomolgus, Rhesus
Antibody Type Monoclonal
Host Species Mouse
Immunogen Human peripheral T cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin
USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under
optimal conditions.
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup
tool if you have a lot number.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric
analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100
µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser Blue Laser (488 nm)
Application Notes The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional
reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and
blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed
paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free,
0.2 μm filtered) is recommended for functional assays (Cat. No. 317404).
In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.
Additional Product Notes BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye
molecule remains the same, so you should expect the same quality and performance from our
PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
(PubMed link indicates
BioLegend citation)
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
Product Citations
Version: 5 Revision Date: 12/05/2018
1. Shi Y, et al. 2014. Proc Natl Acad Sci U S A. 111:3092.. PubMed
2. Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
3. Kongsbak M, et al. 2014. PLoS One. 9:96695. PubMed
4. Jin J, et al. 2014. PLoS One. 9:104753. PubMed
5. Meira M, et al. 2014. Mult Scler. 20:1363. PubMed
6. Florek N, et al. 2014. J Virol. 88:13418. PubMed
7. Crompton J, et al. 2015. Cancer Res. 75:296. PubMed
8. Ma G, et al. 2015. Proc Natl Acad Sci U S A. 112:2216. PubMed
9. Ishigaki K, et al. 2015. Sci Rep. 5: 12937. PubMed
10. Bego M, et al. 2015. J Vis Exp. 103: 51207. PubMed
11. Heery C, et al. 2015. Cancer Immunol Res. 3: 1248 - 1256. PubMed
12. Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed
RRID AB_1186124 (BioLegend Cat. No. 317427)
AB_1186122 (BioLegend Cat. No. 317428)
Antigen Details
Structure Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution T cell subset, majority of thymocytes, monocytes/macrophages
Function MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor MHC class II molecules, HIV gp120, IL-16
Cell Type Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area Immunology
Molecular Family CD Molecules
Antigen References 1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID 920
Related Protocols
Cell Surface Flow Cytometry Staining Protocol
Brilliant Violet 650™ anti-human CD4, Purified anti-human CD4, LEAF™ Purified anti-human CD4, Biotin anti-human CD4, FITC antihuman
CD4, PE anti-human CD4, PE/Cy5 anti-human CD4, PE/Cy7 anti-human CD4, APC anti-human CD4, APC/Cyanine7 anti-human
CD4, Alexa Fluor® 488 anti-human CD4, Alexa Fluor® 647 anti-human CD4, Alexa Fluor® 700 anti-human CD4, Pacific Blue™ antihuman
CD4, PerCP anti-human CD4, Brilliant Violet 421™ anti-human CD4, Brilliant Violet 605™ anti-human CD4, Brilliant Violet
711™ anti-human CD4, Brilliant Violet 785™ anti-human CD4, Brilliant Violet 510™ anti-human CD4, Brilliant Violet 570™ anti-human
CD4, PE/Dazzle™ 594 anti-human CD4, TotalSeq™-A0922 anti-human CD4
Other Formats
Product Data
Human peripheral blood lymphocytes
were stained with CD4 (clone OKT4)
PerCP/Cyanine5.5 (filled histogram) or
Mouse IgG2b, κ PerCP/Cyanine5.5
isotype control (open histogram).
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other
violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written
approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or
commercial use.
BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587
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PerCP/Cyanine5.5 anti-human CD4
¥1190
