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文献和实验Imaging of Mitochondrial pH Using SNARF-1
inside living cells. Here, we describe a confocal microscopic technique to image intracellular pH in living cells using SNARF-1, a ratiometric pH-indicating fluorescent probe. SNARF-1 is ester-loaded into the cytosol and mitochondria of adult cardiac myocytes
Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
that provide optimum conditions for simultaneous measurement of pH from the fluorescence emission of snarf-1, and Ca2+ using fura-2. The fluorescence spectra of these compounds are sufficiently different to allow simultaneous measurement of pH and Ca
(一)BCECF测定pH值 1. 储存液配制 BCECF-AM溶于DMSO配成1mmol/L溶液,等份分装后,-20℃避光保存。 2. 染色步骤 将培养细胞用PBS(含Ca2+、Mg2+)洗2遍。加入BCECF-AM(终浓度1~5μmol/L),37℃孵育30~60min。PBS(含Ca2+、Mg2+)洗2遍。激光扫描共聚焦显微镜观察。 (二)SNARF-1定量比率测定pH值 1. 储存液配制 SNARF溶于DMSO配成1mmol/L溶液,等份分装后,-20℃避光
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