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免疫原:重组小鼠CD40融合蛋白
应用:Flow CytometryFunctional Application (activates B and NK cells in vivo and in vitro)
特异性:特异性识别小鼠CD40
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文献和实验1:1 with Binding buffer.Filter through 0.45 um filters (use prefilters).(2) For Purification of Abs by Protein A columnPrepare Protein A column.Run binding buffer (pH9.0) ~ 200 ml.Filter protein mixtureAdd protein mixture or culture supernatant containing Ab
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
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