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免疫原:重组小鼠CD40融合蛋白
应用:Flow CytometryFunctional Application (activates B and NK cells in vivo and in vitro)
特异性:特异性识别小鼠CD40
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文献和实验1:1 with Binding buffer.Filter through 0.45 um filters (use prefilters).(2) For Purification of Abs by Protein A columnPrepare Protein A column.Run binding buffer (pH9.0) ~ 200 ml.Filter protein mixtureAdd protein mixture or culture supernatant containing Ab
in 100 mM Tris buffer or Binding buffer until pink color is out. Transfer protein mixture (Ab) to 50 ml Falcon tubes to determine volume. Dilute the mixture 1:1 with Binding buffer . Filter through 0.45 um filters (use prefilters
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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