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- 碧云天（beyotime）
- D2207
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
1μg/100μg
| 规格： | 1μg | 产品价格： | ￥888.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1169.0 |
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D2207-1μg | pNFκB-TA-luc (报告基因质粒) | 1μg | 888.00元 |
| D2207-100μg | pNFκB-TA-luc (报告基因质粒) | 100μg | 1169.00元 |
pNFκB-TA-luc (报告基因质粒)是碧云天自行研发的用于检测NFκB转录活性水平的报告基因质粒。pNFκB-TA-luc是以碧云天的pGL6-TA质粒为模板，在其多克隆位点插入了多个NFκB结合位点，可以高灵敏度地检测NFκB的激活水平。
pGL6-TA质粒是用于在哺乳动物细胞中进行萤火虫萤光素酶(firefly luciferase)报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进，一方面对于luciferase的编码进行了改进，确保能更好地在哺乳动物细胞中进行表达，同时对整个质粒中所有的可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理，在保持原有功能不变的情况下，使各种转录因子在质粒上的非特异性结合降到最低。
萤光素、萤光素酶、萤火虫萤光素酶和海肾萤光素酶也经常被称作荧光素、荧光素酶、萤火虫荧光素酶和海肾荧光素酶。
pNFκB-TA-luc质粒的主要信息如下：
| Base pairs | 5651 |
| NFκB response element (κB) | 26-65 |
| Minimal TA promoter (pTA) | 88-110 |
| luc2 reporter gene | 152-1804 |
| SV40 late poly(A) signal | 1839-2060 |
| SV40 early enhancer/promoter | 2108-2526 |
| Synthetic neomycin phosphotransferase | |
| (Neor) coding region | 2551-3345 |
| Synthetic poly(A) signal | 3370-3418 |
| Reporter Vector primer 4 (RVprimer4) binding region | 3485-3504 |
| ColE1-derived plasmid replication origin | 3742 |
| Synthetic Beta-lactamase (Ampr) coding region | 4533-5393 |
| Synthetic poly(A) signal/transcriptional pause site | 5498-5651 |
| Reporter Vector primer 3 (RVprimer3) binding region | 5600-5619 |
pNFκB-TA-luc质粒的图谱如下：
pNFκB-TA-luc的多克隆位点及NFκB response element的详细图谱见说明书。
pNFκB-TA-luc中没有的酶切位点(Restriction enzymes that do not cut pNFκB-TA-luc)包括：
| Aat II | Afl II | Asc I | Ase I | Bsa I | BsaA I | BsiW I | BspM II |
| BssH II | Eco72 I | EcoR I | EcoR V | Mlu I | Nde I | Nru I | PaeR7 I |
| PflM I | Pme I | Pml I | Psp1406 I | PspA I | Rsr II | Sac I | Sma I |
| SnaB I | Spl I | Srf I | Tth111 I | Vsp I | Xcm I | Xho I | Xma I |
pNFκB-TA-luc中的单酶切位点(Restriction enzymes that cut pNFκB-TA-luc once)包括：
| Sfi I | GGCCN,NNN`NGGCC | 9 | BstB I | TT`CG,AA | 3421 | |
| Bgl I | GCCN,NNN`NGGC | 9 | Sal I | G`TCGA,C | 3435 | |
| Acc65 I | G`GTAC,C | 15 | Afl III | A`CRYG,T | 3685 | |
| Asp718 | G`GTAC,C | 15 | ApaL I | G`TGCA,C | 3999 | |
| Kpn I | G,GTAC`C | 19 | HgiE II | ACCNNNNNNGGT -1/13 | 4264 | |
| Nhe I | G`CTAG,C | 21 | Not I | GC`GGCC,GC | 4505 | |
| Bgl II | A`GATC,T | 67 | BstX I | CCAN,NNNN`NTGG | 4529 | |
| Hind III | A`AGCT,T | 77 | BstE II | G`GTNAC,C | 4532 | |
| BsrG I | T`GTAC,A | 643 | Ahd I | GACNN,N`NNGTC | 4607 | |
| Dra III | CAC,NNN`GTG | 1299 | Bsu36 I | CC`TNA,GG | 4963 | |
| Gsu I | CTGGAG 21/19 | 1532 | Pvu I | CG,AT`CG | 4977 | |
| Bpm I | CTGGAG 22/20 | 1533 | Sac II | CC,GC`GG | 5001 | |
| Mun I | C`AATT,G | 1979 | Bst1107 I | GTA|TAC | 5117 | |
| BamH I | G`GATC,C | 2072 | Xca I | GTA|TAC | 5117 | |
| Stu I | AGG|CCT | 2505 | Spe I | A`CTAG,T | 5436 | |
| EcoN I | CCTNN`N,NNAGG | 3026 | BsmA I | GTCTC`/9 | 5448 | |
| BsiC I | TT`CG,AA | 3421 | BsmB I | CGTCTC 7/11 | 5449 |
pNFκB-TA-luc质粒中推荐使用的测序引物序列如下：
RVprimer3(5600-5619):
CTA GCA AAA TAG GCT GTC CC
pNFκB-TA-luc的全序列信息：D2207-pNFkappaB-TA-luc-seq.txt
包装清单：
| 产品编号 | 产品名称 | 包装 |
| D2207-1μg | pNFκB-TA-luc (报告基因质粒) | 1μg |
| D2207-100μg | pNFκB-TA-luc (报告基因质粒) | 100μg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存。
注意事项：
本质粒未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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文献和实验1. Wang YS, Li H, Li Y, Zhu H, Jin YH . Identification of natural compounds targeting Annexin A2 with an anti-cancer effect. Protein Cell. 2018 Jun;9(6):568-579. (IF 10.164)
2. Liu Y, Fang X, Zhang X, Huang J, He J, Peng L, Ye C, Wang Y, Xue F, Ai D, Li D, Zhu Y. . Metabolic profiling of murine plasma reveals eicosapentaenoic acid metabolites protecting against endothelial activation and atherosclerosis. BRIT J PHARMACOL. 2018 Apr;175(8):1190-1204. (IF 7.73)
3. Yuan D, Pan Y, Zhang J, Shao C. . Role of nuclear factor-kappaB and P53 in radioadaptive response in Chang live cells. MUTAT RES-REV MUTAT. 2010 Jun 1;688(1-2):66-71. (IF 5.803)
4. Xu X, Zhang X, Liu Y, Yang L, Huang S, Lu L, Wang S, Guo Q, Zhao L. . BM microenvironmental protection of CML cells from imatinib through Stat5/NF-κB signaling and reversalby Wogonin. ONCOTARGET. 2016 Apr 26;7(17):24436-54. (IF 5.168)
5. Xue H, Gan F, Zhang Z, Hu J, Chen X, Huang K. . Astragalus polysaccharides inhibits PCV2 replication by inhibiting oxidative stress and blocking NF-κB pathway. Int J Biol Macromol. 2015 Nov;81:22-30. (IF 5.162)
6. Guo Z, Chen L, Zhu Y, Zhang Y, He S, Qin J, Tang X, Zhou J, Wei Y. . Double-stranded RNA-induced TLR3 activation inhibits angiogenesis and triggers apoptosis of humanhepatocellular carcinoma cells. Oncol Rep. 2012 Feb;27(2):396-402. (IF 3.417)
7. Chen L, Xu YY, Zhou JM, Wu YY, E Q, Zhu YY. . TLR3 dsRNA agonist inhibits growth and invasion of HepG2.2.15 HCC cells. Oncol Rep. 2012 Jul;28(1):200-6. (IF 3.417)
8. Cheng J, Sun Y, Zhang X, Zhang F, Zhang S, Yu S, Qiu X, Tan L, Song C, Gao S, Wu Y, Ding C. . Toll-like receptor 3 inhibits Newcastle disease virus replication through activation of pro-inflammatory cytokines and the type-1 interferon pathway. Arch Virol. 2014 Nov;159(11):2937-48. (IF 2.243)
9. Wang Y, Wei H, Wang X, Du L, Zhang A, Zhou H. . Cellular activation, expression analysis and functional characterization of grass carp IκBα: evidence for its involvement in fish NF-κB signaling pathway. FISH SHELLFISH IMMUN. 2015 Feb;42(2):408-12. (IF 3.298)
10. Cheng KY, Guo F, Lu JQ, Cao YZ, Wang TC, Yang Q, Xia Q. . MnTM-4-PyP modulates endogenous antioxidant responses and protects primary cortical neurons against oxidative stress. CNS Neurosci Ther. 2015 May;21(5):435-45. (IF 4.074)
11. Hong Y, Zhou L, Xie H, Wang W, Zheng S. . Differences in antiproliferative effect of STAT3 inhibition in HCC cells with versus without HBV expression. BIOCHEM BIOPH RES CO. 2015 Jun 5;461(3):513-8. (IF 2.985)
12. Li DD, Pang HG, Song JN, Huang H, Zhang M, Zhao YL, Sun P, Zhang BF, Ma XD. . The rapid lipopolysaccharide-induced release of matrix metalloproteinases 9 is suppressed by simvastatin. Cell Biol Int. 2015 Jul;39(7):788-98. (IF 2.571)
13. Wang L, Li X, Song YM, Wang B, Zhang FR, Yang R, Wang HQ, Zhang GJ. . Ginsenoside Rg3 sensitizes human non-small cell lung cancer cells to γ-radiation by targeting the nuclear factor-κB pathway. Mol Med Rep. 2015 Jul;12(1):609-14. (IF 2.1)
14. Junmin S, Hongxiang L, Zhen L, Chao Y, Chaojie W. . Ginsenoside Rg3 inhibits colon cancer cell migration by suppressing nuclear factor kappa B activity. J Tradit Chin Med. 2015 Aug;35(4):440-4. (IF 0.676)
15. Xu L, Yu D, Peng L, Fan Y, Chen J, Zheng YT, Wang C, Yao YG. . Characterization of a MAVS ortholog from the Chinese tree shrew (Tupaia belangeri chinensis). Dev Comp Immunol. 2015 Sep;52(1):58-68. (IF 3.192)
16. Zhu Z, Zhao Y, Li J, Tao L, Shi P, Wei Z, Sheng X, Shen D, Liu Z, Zhou L, Tian C, Fan F, Shen C, Zhu P, Wang A, Chen W, Zhao Q, Lu Y. . Cryptotanshinone, a novel tumor angiogenesis inhibitor, destabilizes tumor necrosis factor-α mRNA via decreasing nuclear-cytoplasmic translocation of RNA-binding protein HuR. MOL CARCINOGEN. 2016 Otc;55(10):1399-1410. (IF 3.825)
17. Wei W, Zhang WY, Bai JB, Zhang HX, Zhao YY, Li XY, Zhao SH. . The NF-ҡB modulated miR-195/497 inhibit myoblast proliferation by targeting Igf1r/Insr and cyclin genes. J Cell Sci. 2016 Jan 1;129(1):39-50. (IF 4.573)
18. Jin M, Sun CY, Zang BX. . Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury. Chin J Integr Med. 2016 Jan;22(1):36-41. (IF 1.545)
19. Wu X, Liu P, Zhang H, Li Y, Salmani JM, Wang F, Yang K, Fu R, Chen Z, Chen B. . Wogonin as a targeted therapeutic agent for EBV (+) lymphoma cells involved in LMP1/NF-κB/miR-155/PU.1 pathway. BMC Cancer. 2017 Feb 21;17(1):147. (IF 3.15)
20. Zhai X, Wang L, Xu C, Hou Q, Zhang L, Li Z, Qin W, Liu Z, Chen Z . Triptolide preserves glomerular barrier function via the inhibition of p53-mediated increase of GADD45B. Arch Biochem Biophys. 2019 Aug 15 671:210-217. (IF 3.391)
21. Fei QL, Zhang XY, Qi RJ, Huang YF, Han YX, Li XM, Cai RL, Gao Y, Qi Y . Canscora lucidissima, a Chinese folk medicine, exerts anti-inflammatory activities by inhibiting the phosphorylation of ERK1/2 in LPS-activated macrophages. BMC COMPLEM ALTERN M. 2019 Dec 16 19(1):371. (IF 2.833)
22. Ruijuan Qi, Ximeng Li, Xiaoyu Zhang, Yunfeng Huang, Qiaoling Fei, Yixin Han, Runlan Cai, Yuan Gao, Yun Qi . Ethanol extract of Elephantopus scaber Linn. Attenuates inflammatory response via the inhibition of NF-κB signaling by dampening p65-DNA binding activity in lipopolysaccharide-activated macrophages J Ethnopharmacol. 2020 Mar 25;250:112499. doi: 10.1016/j.jep.2019.112499. (IF 3.69)
