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- 详细信息
- 文献和实验
- 技术资料
- 库存:
咨询
- 英文名:
Agarose for ≧ 1kbp fragment
- 保质期:
三年
- 供应商:
上海科渊生物技术有限公司
- 保存条件:
室温
- 规格:
25g 100g 500g
硫酸根: ≤0.2
凝胶强度: ≥2,500g/cm2(at 1.5%)
凝胶点(℃): 36±1.5
电渗(-mr): 0.09-0.13
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文献和实验Mesoplasma florum:Inverse PCR Transposon location
frequency calculation: p(cut) = (.13)(.37)(.37)(.13) = .00231 Expected fragment length = 1/ .00231 = 432 bp + length from primer site to end of transposon Expected PCR fragment length is twice this length, or about 1Kbp Materials
by chromatography on a size exclusion column such as Sephacryl S-500. DNA Extraction from Agarose Gel DNA Fragment Purification from Agarose or Acrylamide (Mike A. Dyer) For fragments from 200 bp to 10 kb the agarose purification
5 ) or 1.5% agarose gel (GAPDH ) and quantified using ImageQuant 1.2. The 1-kbp CDH5 -specific PCR product was sequenced and shown to correspond to the expected CDH5 sequence. Luciferase assays. The human CDH5 promoter fragment (-2486 to +24) was amplified
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