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- 详细信息
- 文献和实验
- 技术资料
- 库存：
咨询
- 英文名：
Agarose for ≧ 1kbp fragment (Fine Powder)
- 保质期：
三年
- 供应商：
上海科渊生物技术有限公司
- 保存条件：
室温
- 规格：
10g 100g 500g
» 粉末状，称量方便
» 电泳条带鲜明清晰
规格参数
型号 : ≧ 1kbp
硫酸根 (%) : ≦ 0.2
凝胶强度 : ≧ 2,500 g/cm2 (at 1.5%)
凝胶点 (oC) : 36 ± 1.5
电渗 (-mr) : 0.09-0.13
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文献和实验is the more familiar state of seaweed, as far as molecular biologists are concerned. As mentioned before, high grade agarose is expensive. Guess how much this 500 gram bottle costs. Would you believe $200?!? Now it's time to learn what we do with this powder
. ANALYTIC AGAROSE GEL Determine DNA concentration and yield on agarose gel. (Assume 50% loss and load corresponding amount of nebulized lambda DNA as a standard.VI. LIGATION INTO VECTOR AND TRANSFORMATION 335 ng insert DNA (for medium size of 1 kbp
Organelle DNA Library Construction
down. III. SIZING 1. Separate on LM agarose gel (1ug/0.5-cm well) and electro-elute 2) fractions (0.5 - 1.0 kbp and 1.0 - 3 kbp; ligate separately) 2. Add glycogen (1 ul of 20mg/ml stock), precipitate by EtOH/AmAc (spin
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