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- 详细信息
- 文献和实验
- 技术资料
- 库存:
15瓶
- 保质期:
12个月
- 供应商:
上海科渊生物技术有限公司
- 保存条件:
-20℃
• High performance medium for iPSCs • Manufactured under GMP compliance • Suitable for clinical research applications • Meets Japan regulatory materials standards • Backed by REPROCELL’s quality and experience
Overview ReproMed iPSC Medium is formulated and designed for cultivation of iPS cells destined for cell therapy applications. In close consultation with regulatory authorities we have formulated a medium with components meeting the strict purity and traceability standards of Japan. The product has been manufactured in GMP-compliant facilities for added safety and reproducibility. ReproMed iPSC Medium is validated for tri-lineage pluripotency by in vitro differentiation into mesoderm, endoderm and ectoderm cell lineages. Backed by over a decade of quality and experience, REPROCELL is a brand you can trust.
Fiigure 1. ReproMed™ iPSC Medium supports robust growth of multiple iPSC lines. For preparing complete medium, supplementation of ReproMed™ with basic FGF is required to 10 ng/mL. The complete medium provides robust growth and expansion for iPSC regardless of the reprogramming method employed (253G1, 201B7 iPSC were reprogrammed using retrovirus by Kyoto Univ.; RPChiPS771 iPSC was reprogrammed using mRNA by Stemgent). The ReproMed growth data demonstrates 500× to 1000× fold expansion in suspension culture in 3 weeks, out-performing another leading brand of medium.
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文献和实验-integrating reprogramming method and in culture conditions that are completely absent of animal proteins will be ideal for such regenerative and cell therapy applications. Here we describe a method to generate non-integrating iPSCs using the Episomal iPSC
This chapter describes a protocol for deriving induced pluripotent stem cells (iPSCs) from human fibroblasts. Human fibroblasts, cultured in fibroblast medium, are infected with a cocktail of retroviral vectors expressing the transcription
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
the medium to iPSC medium, and replace the spent medium everyday thereafter. b. Starting on Day 8, observe the plates every other day under a microscope for the emergence of cell clumps indicative of transformed cells. Note: For BJ











