Alkaline phosphatase, tissue-n
onspecific isozyme Recombinant Rabbit mAb(bsm-52252R)-50ul/100ul/200ul/200ug/25ul- ￥800 - 5600
- Bioss
- bsm-52252R
- 2025年10月24日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul/200ug/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 200ul | 产品价格： | ￥4000.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-52252R |
| 英文名称 | Alkaline phosphatase, tissue-nonspecific isozyme Recombinant Rabbit mAb |
| 中文名称 | 碱性磷酸酶重组兔单抗 |
| 英文别名 | ALPL; AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. |
| 中文别名 | 非组织特异性碱性磷酸酶; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Alkaline phosphatase, tissue-nonspecific isozyme |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 56 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homodimer. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 翻译后修饰 | Glycosylated. |
| 相似性 | Belongs to the alkaline phosphatase family. |
| 功能 | This isozyme may play a role in skeletal mineralization. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
Blocking of unwanted non-specific staining
that may affect your staining. Blocking of endogenous Alkaline Phosphatase is done by exploiting the differential sensitivity of calf intestinal AP, used as a reporter, and leukocyte AP. This latter is inhibited, while the former is not by Levamisole 1 mM
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