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- bsm-0295M-HRP
- 2025年10月10日
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100ul/1ml
| 规格： | 100ul | 产品价格： | ￥240.0 |
|---|---|---|---|
| 规格： | 1ml | 产品价格： | ￥1580.0 |
| 产品编号 | bsm-0295M-HRP |
| 英文名称 | Mouse Anti-Rabbit IgG mAb, HRP conjugated |
| 中文名称 | HRP标记小鼠抗兔IgG H&L单克隆抗体 |
| 英文别名 | Mouse Anti rabbit IgG mAb (HRP); Immunoglobulin G; |
| 产品应用 | WB=1:1000-10000, ELISA=1:1000-10000, IHC-P=1:100-1000, IHC-F=1:100-1000 Not yet tested in other applications. |
| 交叉反应 | Rabbit |
| 抗体来源 | Mouse |
| 免疫原 | Native Rabbit IgG |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {ELISA} | {1:1000-10000} |
| {IHC-P} | {1:100-1000} |
| {IHC-F} | {1:100-1000} |
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文献和实验[IF={{ 7.7 }}] {Zhineng Liu. et al.Proline rich-39 (PR-39) antimicrobial protein alleviated lipopolysaccharide-induced intestinal barrier dysfunction in piglets by altering intestinal flora associated bile acid metabolism and in turn regulating TGR-5/NF-κB/MLCK/MLC pathway..International Journal of Biological Macromolecules.2025 Mar 10;307(Pt 1):141930.} {IF} {Human}
[IF={{ 5.6 }}] {Liping Liu. et al.IL-33/ST2 signalling promotes tumor growth by regulating polarization of alternatively activated macrophages..Cancer Biology & Medicine.2025 Mar 27:j.issn.2095-3941.2024.0483.} {Western blot} {Mouse}
[IF={{ 5 }}] {Dengliang Li. et al. Lactobacillus acidophilus protects against Corynebacterium pseudotuberculosis infection by regulating the autophagy of macrophages and maintaining gut microbiota homeostasis in C57BL/6 mice. MSYSTEMS. 2024 六月 27} {WB} {Mouse}
[IF={{ 3.2 }}] {Xiu Min Zhang. et al. Low-protein diet promotes nitrogen retention efficiency via enhanced renal urea reabsorption and microbial hydrogen incorporation in the rumen of goats. ANIM FEED SCI TECH. 2023 Sep;:115762} {WB} {Goat}
[IF={{ 2.145 }}] {Wenxun Chen. et al. Effects of maternal feed intake restriction on the blood parameters, fatty acid profile and lipogenetic genes expression of perirenal fat in offspring kids. Anim Reprod Sci. 2022 Mar;238:106955} {WB} {Goat}
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
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