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- bsm-0297M-PerCP-Cy7
- 2025年10月10日
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100ul
| 产品编号 | bsm-0297M-PerCP-Cy7 |
| 英文名称 | Mouse Anti-Human IgG H&L (3D3) mAb, PerCP-Cy7 conjugated |
| 中文名称 | PerCP-Cy7标记的鼠抗人IgG H&L单克隆抗体 |
| 英文别名 | Immunoglobulin G (IgG); hu IgG; human Immunoglobulin G; hu Immunoglobulin G. |
| 产品应用 | ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:100-1000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | Native Human IgG |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1:100-1000} |
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
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