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- 文献和实验
- 技术资料
- 保存条件:
Liquid nitrogen
- 库存:
100
- 规格:
1 vial
Dry Ice
存储温度:
Liquid nitrogen
检测方法:
N/A
类别:
Cell Line
产品分类:
Stem Cell Research-> Feeder Cells->MEF Feeder Cells
产品描述:
MEF Feeder Cells are mouse embryonic fibroblasts that allow stem cell culture without LIF. Cells must be mitotically inactivated prior to addition of stem cells. Hygromycin resistant.
联系方式:
生物商城:http://www.annoron.com
办公电话:010-56256916
官方售后:400-965-8633
企业 QQ:3212874516、3198592576
地 址:北京经济技术开发区永昌中路4号院4号楼3层
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs
Propagation of Human Embryonic Stem Cells on Human Feeder Cells
on human fibroblast feeder cells. Two culture methods, the bulk enzymatic culture protocol and the microdissection “cut and paste” protocol are described. Only certain human fetal and adult fibroblast feeders support hES cell growth. Methods
Feeder Layers: Co-culture with Nonneoplastic Cells
Maintenance of a mitotically inactive feeder layer which is able to provide extracellular matrix and growth factors can be critical in establishing and maintaining primary tumor cells. How feeder cells are handled and processed is crucial
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