- ￥4980.18
- nsjbio
- R30169
- 2025年12月06日
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Amino acids 21-35 (EKEKDPKYWRDQAQE) of human ALPL were used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.
- 亚型：
Rabbit IgG
- 适应物种：
Human, Mouse, Rat
- 宿主：
Rabbit
- 规格：
100 ug
Primary antibody
纯化方法：
Antigen affinity purified
通用信息：
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
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