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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
2年
- 英文名:
T4 RNA Ligase 1
- 库存:
充足
- 供应商:
广州英赞生物科技有限公司
【产品概述】
本酶用于催化 3´→5´磷酸二酯键的形成,使核苷酸的 5´ -磷酸末端和 3´ -羟基末端连接,伴随着 ATP 水解为 AMP 和 PPi。作用底物包括单链 RNA、DNA 及二核苷焦磷酸。
【浓度】10 U/μL
【来源】
来自重组 E. coli 菌株,携带有T4 RNA Ligase 1片段编码基因。
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文献和实验Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein interactions are increasingly using site-specifically modified RNAs to probe sites of interest (1 ). In addition to expanding the repertoire of functional groups
Splint Ligation of RNA with T4 DNA Ligase
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase
Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
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