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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
2年
- 英文名:
PreScript One-Step RT-PCR Kit
- 库存:
充足
- 供应商:
广州英赞生物科技有限公司
- 规格:
S035A:50rxn S035B;200rxn
一步法RT-PCR扩增试剂盒
产品概述
产品内容
| 组分 | S035A | S035B |
| Prescript One-Step Enzyme Mix | 100 μL | 400 μL |
| 2× One-Step RT-PCR Buffer | 625 μL × 2 | 1.25 mL× 2 |
| Control F-1 Primer(20 μM) | 20 μL | 80 μL |
| Control R-1 Primer(20 μM) | 20 μL | 80 μL |
| Positive Control RNA | 20 μL | 80 μL |
| RNase Free ddH2O | 625 μL× 2 | 1.25 mL× 2 |
产品优势
扩增能力强:该试剂盒扩增长至3 kb基因片段,产物可用于TA克隆
检测灵敏度高:可检测低至10 pg的Total RNA,满足RNA检测的需求
操作简便:一管式反应,很大程度减少移液次数,避免操作失误 及交叉污染
实验案例
1. 扩增能力及灵敏度
应用范围
一步法RT-PCR、病毒RNA检测、TA克隆
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文献和实验This protocol uses C. therm. Polymerase One-Step RT-PCR system from Roche. 1. Setup master mix 1: 25 mM dNTP 0.8 m l DMSO
Two-Step RT-PCR protocol We will provide both one-step and two-step protocols for RT -PCR . We recommend the two-step protocol for this class. In the one-step protocol, the components of RT and PCR are mixed in a single tube
using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates










