- ¥508
- Invitrogen™ eBioscience™
- 00-4201-56
- 2025年07月30日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Invitrogen™ eBioscience™ TMB Solution (1X) 00-4201-56
- 供应商：
上海辅泽商贸有限公司
- 规格：
100 mL
eBioscience™ TMB Solution (1X)
货号： 00-4201-56
| 货号 | 单位规格 | 单价 (CNY) | 数量 | |
|---|---|---|---|---|
| 00-4201-56 | 100 mL | 508.00 |
规格
| Detection Method: | Colorimetric |
|---|---|
| Product Line: | eBioscience™ |
| Product Size: | 100 mL |
| Substrate Properties: | Chemical Substrate |
| Substrate Type: | HRP (Horseradish Peroxidase) Substrate |
描述
The 1x eBioscience TMB Solution is a sensitive chromogenic substrate supplied as single ready-to-use solution. TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for Ready-SET-Go! ELISA kits.During the enzymatic reaction the TMB substrate will result in a blue color that can be read on a spectrophotometer at 650 nm. This kinetic reaction can be stopped using 1M phosphoric acid resulting in a color change to yellow that can be read at 450 nm.
Reported Application
ELISA
For Research Use Only. Not for use in diagnostic procedures.
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A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay
a background and then counted as specific spots. We have tested a cell detachment reagent, Accumax™, and found that it may be used to remove a large number of cells adhered to the microplate membranes. Accumax was tested in 16 different ELISPOT assays
genomic DNA elute/ modified lysis buffer solution (15 minutes at R/T under constant motion);5.Following the 15 minute incubation the beads are captured using a magnetic bead separator (Invitrogen™) and washed 3 times with 2x wash buffer
