Certified Fetal Bovine Serum,
Qualified for Human Embryonic Stem Cells 金牌胚胎干细胞专用胎牛血清 04-002-1A- ¥4800
- BI
- 04-002-1A
- 以色列
- 2026年02月28日
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- 保存条件：
-20度
- 规格：
04-002-1A
货号：04-002-1A
规格：500ml
产品名称：Certified Fetal Bovine Serum, Qualified for Human Embryonic Stem Cells
Product Overview
Embryonic Stem (ES) Cells are pluripotent cells derived from the inner cell mass of the blastocyst. The stem cells can be maintained in vitro for extended periods without loss of their capacity to differentiate to all cell lineages when reimplanted back into a blastocyst. ES cells may differentiate in vitro to a variety of cell types including neuronal, muscle, endothelial and hematopoietic progenitors. General culture conditions are well established and usually require ES cells to be grown on an inactive feeder cell layer or with basement membrane components (Matrigel, Fibronectin, Laminin). When growing ES cells, one of the most important parameters is the maintenance of the cells in the undifferentiated state. Pre-screening of the serum is essential before using it for the culture of ES cells. Various lots of sera are screened for the growth of Human ES cells using MEF's as a feeder layer.
The following parameters are measured for the screening:
Human ES cells colony morphology
Plating efficiency
Differentiation rate: analysis of Human ES cells surface marker expressed on the undifferentiated cells membrane (FACS analysis).
The results are used as an indication of the quality of sera for the growth and maintenance of undifferentiated stem cells.
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文献和实验Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent cells derived from the embryo at the blastocyst stage. Their embryonic origin confers upon them the capacity to proliferate indefinitely in vitro while maintaining the capacity
Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
, isolation and culturing were traditionally conducted on mouse embryonic fibroblast feeder layers, using medium supplemented with fetal bovine serum. However, these conditions allow possible exposure of the cells to animal pathogens. Because both research
Feeder-Layer Free Culture System for Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent stem cells derived from the inner cell mass of the blastocyst. Due to their unique properties, hESCs might be used for research fields such as self-renewal, specific lineage differentiation
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