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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性:
单克隆
- 适应物种:
Human
- 库存:
100
- 宿主:
Mouse
- 应用范围:
WB, IHC-P, E, IHC-Fr, Flo
- 靶点:
Reacts with colon gastric cancer cells, normal intestine, colon and salivary gland (immunoperoxidase staining); human colon cancer cell line LS174T by flow cytometry & Western blotting. Epitope defined as -GTQTP- (GlyThrGlnThrPro).
- 规格:
50 μg
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
to agarose beads in chromatin samples prior to antibody reaction. (17) Pellet the agarose beads by brief centrifugation (3000-5000 x g (ex. 6600 rpm at Eppendorf table top centrifuge) for 1 min)). (18) Collect supernatants into new tubes. Remove
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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