- $395
- Leading Biology
- AMM01453G
- 2025年09月23日
- IF, FC
- Mouse
- Human, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG2a, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human, Monkey
- 库存：
100
- 宿主：
Mouse
- 应用范围：
IF, FC
- 规格：
50 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
ELISA Procedure for Measuring Serum Antibody Titer
reader Pipette tips Solutions to Prepare for the ELISA ProcedureSodium carbonate (Na2CO3) buffer, 50 mM, pH 9.6 with 0.02% NaN3 PBS-T (PBS containing 0.05% Tween-20) 3% BSA in PBS-T Sodium acetate (NaOAc), 0.1 M ELISA Procedure Run duplicates
- Blocking solution = 2 BSA (type V ) in PBS. (Add 0.02 azide for longer storage.) - Elisa buffer = 2 BSA + 0.1 Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish
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