- $395
- Leading Biology
- AMM00410G
- 2025年09月21日
- IF, FC
- Mouse
- Human, Mouse, Rat, Cow
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human, Mouse, Rat, Cow
- 库存：
100
- 宿主：
Mouse
- 应用范围：
IF, FC
- 规格：
50 ug
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Replication timing by density transfer
), arrest with 200 nM alpha factor (1-1.25 doubling times, until % unbudded cells stops increasing). NOTE: This alpha factor concentration is for bar1 strains. BAR1+ strains require 15x more alpha factor. 3. Filter, wash in Ycomp+alpha factor, resuspend
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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