- $395
- Leading Biology
- AMM00120G
- 2025年09月21日
- WB, IHC, IF, FC, IP, E
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 库存：
100
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF, FC, IP, E
- 靶点：
Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose
- 规格：
50 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
ELISA Procedure for Measuring Serum Antibody Titer
of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent
Antibody Staining of Dissected Gonads
Antibody Staining of Dissected Gonads - From R. Francis - -Adapted by Min-Ho Lee- Antibody staining (in Tubes) 1. Using a pasteur pipette (drawn in flame and cut open), transfer worm carcasses with attached gonads
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