Ready-to-use 3D Human Chondrocyte-articular Spheroid Kit说明:
The composition and structural integrity of extracellular matrix (ECM) within the cartilage is responsible for enduring the tensile strength of joint biomechanics. Chondrocytes, the main constituent of cartilage, mediate the synthesis and degradation of ECM macromolecules (such as Type II collagen and aggrecan) within the matrix. Type II collagen and aggrecan provide the tensile strength and the osmotic resistance for cartilage, respectively. Chondrocytes in monolayer culture, however, are susceptible to dedifferentiation. Thus, an improved cell culture model that closely mimics the in vivo environment is necessary to maintain the features of differentiated chondrocytes. ScienCell has developed a highly innovative ready-to-use 3D chondrocyte spheroid kit (SP3D-HCaS) that better approximates the in vivo environment. Using ScienCell’s Ready-to-use 3D spheroid kit, researchers can obtain highly homogenous and functional 3D spheroids in 24 hours after thawing, without encountering the long and complex workflow of 3D culture.
In 3D spheroid culture, we confirmed that the chondrocytes maintain functional markers such as type II collagen, aggrecan, and Sox9 as determined by qPCR analysis. ScienCell’s SP3D-HCaS provides an excellent in vitro model for studying normal chondrocyte physiology, mechanism of degenerative joint diseases, and cartilage tissue repair and engineering. 软骨细胞外基质(ECM)的组成和结构完整性是关节生物力学抗拉强度持久的基础。软骨细胞是软骨的主要组成部分,介导基质内ECM大分子(如II型胶原和aggrecan)的合成和降解。型胶原和骨胶原分别提供软骨的抗拉强度和渗透阻力。然而,单层培养的软骨细胞易发生去分化。因此,为了维持分化软骨细胞的特征,需要一种与体内环境高度相似的改良细胞培养模型。ScienCell已经开发了一种高度创新的现成的3D软骨细胞椭球体试剂盒(SP3D-HCaS),可以更好地模拟体内环境。利用ScienCell随时可用的3D椭球体试剂盒,研究人员可以在解冻后24小时内获得高度同质、功能齐全的3D椭球体,而不需要面对漫长而复杂的3D培养工作流程。 在三维椭球体培养中,我们通过qPCR分析确定软骨细胞保持了II型胶原、aggrecan和Sox9等功能标记物。ScienCell的SP3D-HCaS为研究正常软骨细胞生理、退行性关节疾病机制、软骨组织修复和工程提供了一个极好的体外模型。
Catalog No.
SP3D-4650
Country of Manufacture
United States
Product Code
SP3D-HCaS
Size/Quantity
Product Use
SP3D-HCaS is for research use only. It is not approved for human or animal use, or application in clinical or in vitrodiagnostic procedures.
Storage
Shipping Info
SP-4650, 3D-4682, 0010, and 0583 are shipped on dry ice. 3D-4651, and [0343 (or) 0353 (or) 0383] are shipped at room temperature.