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- Thermo Scientific
- M1631
- 2026年01月02日
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产品描述
Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.
Highlights
• Fast—short reaction time (typically 30–60 min)
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reaction
Applications
First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
规格
| Reverse Transcriptase: | ThermoScript™ |
|---|---|
| Downstream Application: | PCR, Real-Time PCR |
| Reaction Format: | SuperMix or Master Mix |
| Sample Type (General): | Cells |
| Product Line: | RevertAid |
| Product Size: | 100 reactions |
| Shipping Condition: | Dry Ice |
内容及储存
• 2 x 50 µL of 20X Enzyme mix (MuLV and RNase inhibitor protein)
• 2 x 500 µL of 2X RT Buffer Mix (includes dNTPs, random octamers, and Oligo dT-16)
Store at -15 to -25°C. 2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.
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文献和实验Invitrogen 性能卓越的系统整合了可以利用的最好的RT技术，包括SuperScript™ III RT和最新推出的Thermo-X™ RT。SuperScript™ III RT是M-MLV RT基因工程升级版本，减少了RNaseH活性，提供更高的热稳定性（1-3）。这种酶可以在高达55℃的情况下合成cDNA，比以前使用的反转录酶提供更高的特异性，更高的cDNA产量以及更多的全长产物。Thermo-X™ RT克隆自一种嗜热真细菌，提供高达70℃的热稳定
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
#2 Forward Master Mix”,etc. 6.Using the table below,each group will prepare master mixes for each DNA template with primer to be sequenced.Begin by adding the water first,Reaction Buffer,DNA and primer,and Thermo Sequenase polymerase last.**Important
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