- ￥5673
- Invent
- BN-020
- 美国
- 2026年04月24日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells
- 供应商：
英文特生物技术（北京）有限公司
- 保存条件：
4℃
- 规格：
20 Preps
MinuteTM 神经元组织/细胞单个细胞核分离试剂盒
目录号：BN-020
描述：
分离的细胞核可广泛应用于FACS分析、单核分析（如RNA-seq和ATAC-seq）、免疫荧光染色、细胞周期分析和凋亡研究等多种实验。单细胞RNA测序是研究组织复杂组成的有力技术。然而，神经元是高度互联的，很难从神经元组织中，例如脑和脊髓等获得单个细胞。从冷冻的神经元组织中分离出完整的细胞更加困难。由于这些限制，因此单细胞RNA测序可以使用单核测序取代。从神经元组织中分离单个核的传统方法比较繁琐、耗时长，得率低，且很难去除髓鞘和其他细胞成分的污染。本试剂盒专门为解决以上问题而设计，操作简单、快速、且分离效率高。与传统方法相比，该试剂盒所需的起始原料更少，可处理1-25mg范围的样品。缓冲液中含有专用的表面活性剂，可有效的溶解细胞。如需要不含有表面活性剂的完整细胞核分离，可以选择cat#NI-024。
应用：
分离的细胞核可用于单个细胞核分析（RNA-seq和ATAC-seq）,流式细胞仪分析，染色体免疫共沉淀（芯片），免疫荧光染色。
参考文献：
1.Klein, K. M., Mascarenhas, R., Merrikh, D., Khanbabaei, M., Maroilley, T., Kaur, N., ... & Tarailo-Graovac, M. (2024). Identification of a novel mosaic MTOR variant in purified neuronal DNA from depth electrodes in a patient with focal cortical dysplasia. medRxiv, 2024-01.
2.Wojick, J. A., Paranjapye, A., Chiu, J. K., Mahmood, M., Oswell, C., Kimmey, B. A., ... & Corder, G. (2024). A nociceptive amygdala-striatal pathway for chronic pain aversion. bioRxiv, 2024-02.
3.James, J. G., McCall, N. M., Hsu, A. I., Oswell, C. S., Salimando, G. J., Mahmood, M., ... & Corder, G. (2024). Mimicking opioid analgesia in cortical pain circuits. bioRxiv, 2024-04.
4.Vornholt, E., Liharska, L. E., Cheng, E., Hashemi, A., Park, Y. J., Ziafat, K., ... & Beckmann, N. D. (2024). Characterizing cell type specific transcriptional differences between the living and postmortem human brain. medRxiv, 2024-05.
5Guo, X., Keenan, B. T., Reiner, B. C., Lian, J., & Pack, A. I. (2024). Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation. Cell Reports, 43(5).
6.Rodriguez de los Santos, M., Kopell, B. H., Buxbaum Grice, A., Ganesh, G., Yang, A., Amini, P., ... & Breen, M. S. (2024). Divergent landscapes of A-to-I editing in postmortem and living human brain. medRxiv, 2024-05.
7.Kalef-Ezra, E., Friscioni, L., Horner, D., Morley, C., Morrow, G., Guo, Y., & Proukakis, C. (2024). Fluorescence-activated nuclei sorting (FANS) for single-cell Whole Genome Sequencing (scWGS).
8.Tyshkovskiy, A., Kholdina, D., Ying, K., Davitadze, M., Molière, A., Tongu, Y., ... & Gladyshev, V. N. (2024). Transcriptomic Hallmarks of Mortality Reveal Universal and Specific Mechanisms of Aging, Chronic Disease, and Rejuvenation. bioRxiv, 2024-07.
9.Mascarenhas, R., Merrikh, D., Khanbabaei, M., Kaur, N., Ghaderi, N., Maroilley, T., ... & Klein, K. M. (2024). Detecting somatic variants in purified brain DNA obtained from surgically implanted depth electrodes in epilepsy. medRxiv, 2024-07.
10.Rodrigues-Amorim, D., Bozzelli, P. L., Kim, T., Liu, L., Gibson, O., Yang, C. Y., ... & Tsai, L. H. (2024). Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice. Nature Communications, 15(1), 6744.
11.McSwiggin, H., Wang, R., Magalhaes, R. D. M., Zhu, F., Doherty, T. A., Yan, W., & Jendzjowsky, N. (2024). Comprehensive sequencing of the lung neuroimmune landscape in response to asthmatic induction. bioRxiv, 2024-10.
12.Brian H. Kopell; Deepak Kaji; Lora E. Liharska; Eric Vornholt; Alissa Valentine; et al.(2024).Multiomic foundations of human prefrontal cortex tissue function.medRxiv (Cold Spring Harbor Laboratory).DOI 10.1101/2024.05.17.24307537
13.Hashimoto S, Nagoshi N, Shinozaki M, Nakanishi K, Suematsu Y, Shibata T, Kawai M, Kitagawa T, Ago K, Kamata Y, Yasutake K, Koya I, Ando Y, Minoda A, Shindo T, Shibata S, Matsumoto M, Nakamura M, Okano H. Microenvironmental modulation in tandem with human stem cell transplantation enhances functional recovery after chronic complete spinal cord injury. Biomaterials. 2023 Apr;295:122002. doi: 10.1016/j.biomaterials.2023.122002. Epub 2023 Jan 26. PMID: 36736008.
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- 作者
- 内容
- 询问日期
文献和实验1.Klein, K. M., Mascarenhas, R., Merrikh, D., Khanbabaei, M., Maroilley, T., Kaur, N., ... & Tarailo-Graovac, M. (2024). Identification of a novel mosaic MTOR variant in purified neuronal DNA from depth electrodes in a patient with focal cortical dysplasia. medRxiv, 2024-01.
2.Wojick, J. A., Paranjapye, A., Chiu, J. K., Mahmood, M., Oswell, C., Kimmey, B. A., ... & Corder, G. (2024). A nociceptive amygdala-striatal pathway for chronic pain aversion. bioRxiv, 2024-02.
3.James, J. G., McCall, N. M., Hsu, A. I., Oswell, C. S., Salimando, G. J., Mahmood, M., ... & Corder, G. (2024). Mimicking opioid analgesia in cortical pain circuits. bioRxiv, 2024-04.
4.Vornholt, E., Liharska, L. E., Cheng, E., Hashemi, A., Park, Y. J., Ziafat, K., ... & Beckmann, N. D. (2024). Characterizing cell type specific transcriptional differences between the living and postmortem human brain. medRxiv, 2024-05.
5Guo, X., Keenan, B. T., Reiner, B. C., Lian, J., & Pack, A. I. (2024). Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation. Cell Reports, 43(5).
6.Rodriguez de los Santos, M., Kopell, B. H., Buxbaum Grice, A., Ganesh, G., Yang, A., Amini, P., ... & Breen, M. S. (2024). Divergent landscapes of A-to-I editing in postmortem and living human brain. medRxiv, 2024-05.
7.Kalef-Ezra, E., Friscioni, L., Horner, D., Morley, C., Morrow, G., Guo, Y., & Proukakis, C. (2024). Fluorescence-activated nuclei sorting (FANS) for single-cell Whole Genome Sequencing (scWGS).
8.Tyshkovskiy, A., Kholdina, D., Ying, K., Davitadze, M., Molière, A., Tongu, Y., ... & Gladyshev, V. N. (2024). Transcriptomic Hallmarks of Mortality Reveal Universal and Specific Mechanisms of Aging, Chronic Disease, and Rejuvenation. bioRxiv, 2024-07.
9.Mascarenhas, R., Merrikh, D., Khanbabaei, M., Kaur, N., Ghaderi, N., Maroilley, T., ... & Klein, K. M. (2024). Detecting somatic variants in purified brain DNA obtained from surgically implanted depth electrodes in epilepsy. medRxiv, 2024-07.
10.Rodrigues-Amorim, D., Bozzelli, P. L., Kim, T., Liu, L., Gibson, O., Yang, C. Y., ... & Tsai, L. H. (2024). Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice. Nature Communications, 15(1), 6744.
11.McSwiggin, H., Wang, R., Magalhaes, R. D. M., Zhu, F., Doherty, T. A., Yan, W., & Jendzjowsky, N. (2024). Comprehensive sequencing of the lung neuroimmune landscape in response to asthmatic induction. bioRxiv, 2024-10.
12.Brian H. Kopell; Deepak Kaji; Lora E. Liharska; Eric Vornholt; Alissa Valentine; et al.(2024).Multiomic foundations of human prefrontal cortex tissue function.medRxiv (Cold Spring Harbor Laboratory).DOI 10.1101/2024.05.17.24307537
13.Hashimoto S, Nagoshi N, Shinozaki M, Nakanishi K, Suematsu Y, Shibata T, Kawai M, Kitagawa T, Ago K, Kamata Y, Yasutake K, Koya I, Ando Y, Minoda A, Shindo T, Shibata S, Matsumoto M, Nakamura M, Okano H. Microenvironmental modulation in tandem with human stem cell transplantation enhances functional recovery after chronic complete spinal cord injury. Biomaterials. 2023 Apr;295:122002. doi: 10.1016/j.biomaterials.2023.122002. Epub 2023 Jan 26. PMID: 36736008.
14.Blake A. Kimmey; Lindsay L. Ejoh; Lily Shangloo; Jessica A. Wojick; Samar N. Chehimi; et al.(2025).Convergent state-control of endogenous opioid analgesia.bioRxiv.DOI 10.1101/2025.01.03.631111
15.Nicha Puangmalai; Abbigael E Aday; Madison Samples; Nemil Bhatt; Filippa Lo Cascio; et al.(2025).Pathogenic Oligomeric Tau alters Neuronal RNA Processes through the Formation of Nuclear Heteromeric Amyloids with RNA-Binding Protein Musashi1.Progress in Neurobiology.DOI 10.1016/j.pneurobio.2025.102742
16.Fernández, G.;López-González, L.; Pons-Fuster, E.;Puelles, L.; Garcia-Calero, E.(2025).Transcriptomic Analysis Corroborates the New Radial Model of the Mouse Pallial Amygdala. Biomolecules 2025,15, 1160.
17.Masataka Kikuchi; Akinori Miyashita; Yu Hirota; Norikazu Hara; Mai Hasegawa; et al.(2025). Microglia-specific polygenic risk stratification reveals distinct pathological subtypes in Alzheimer’s disease.medRxiv.DOI 10.1101/2025.10.06.25336925
18. Anika Nabila; Rose Sciortino; Nicole Politowska; Elizabeth Brindley; Peter U. Hámor; et al.(2025).Adverse gestational environment configures a subpopulation of ventral dentate granule cells for recruitment to drive innate anxiety.Cell Reports.DOI 10.1016/j.celrep.2025.116219
19.McSwiggin H, Wang R, Magalhães RDM,Zhu F, Doherty TA, Yan W and Jendzjowsky N.(2025) Comprehensive sequencing of the lung neuroimmune landscape in response to asthmatic induction.Front. Immunol. 16:1518771.doi: 10.3389/fimmu.2025.1518771
Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
表达水平是正常 人脑脊液的 5-9 倍，大幅提升了治疗的安全窗口。相比传统 AAV9 载体，AAV-EP+展现出更好的安全特征，包括极低的外周组织 转导（肝脏、心脏），背根神经节转导率 图 9 AAV-Ep+在成年食蟹猴中的感染效果（Sci Transl Med，2025，17, eadr2531） rAAV 在神经科学研究中的应用案例 实例一：全脑细胞类型特异性稀疏神经元标记方法及其在 Shank3 自闭症小鼠模型中的应用 单个神经元作为大脑的基本单位，由细胞体和突起组成，包括树突和轴突。即使是同一
酶及其作用 （5）主要实验流程 2.组织类型决定制备方案的差异 （1）方案差异化的决定因素 细胞外基质（ECM）的成分与密度：例如，纤维化肿瘤的胶原蛋白含量远高于脑组织。 细胞间连接的紧密程度：上皮组织的紧密连接比淋巴组织要牢固得多。 组织内源性酶的含量：胰腺等组织富含内源性蛋白酶，解离时易发生自溶。 目标细胞类型及其敏感性：神经元等细胞对酶解和机械力非常敏感，而肿瘤细胞通常较为耐受。 （2）一张表快速了解不同组织类型 神经组织的单细胞制备是所有组织类型中最具挑战性的领域
