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- 英文名:
Pierce™ Primary Neuron Isolation Kit
- 库存:
需确认
- 供应商:
嵘崴达
- CAS号:
见产品外包装
- 规格:
1 L
Pierce™ 原代神经元分离试剂盒
货号: 88280
The Thermo Scientific Pierce Primary Neuron Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary neurons from embryonic cortical and hippocampal tissue from mouse and rat.
Features of the Primary Neuron Isolation Kit:
• Optimized—procedure and reagents optimized for viability, yield, purity, and ease-of-use
• Yield—provides a 2-fold increase in yield compared to do-it-yourself methods
• Viability—greater than 95% of isolated cells remain viable in culture from Day 1 to Week 4
• Functional—cultured neurons contain processes indicative of complete differentiation and functionality
The Primary Neuron Isolation Kit has been designed to obtain high viability and purity of primary embryonic neurons using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains tissue-specific dissociation enzymes, media formulated specifically for neuronal cell culture, and cell culture supplements that promote the isolation and growth of functional neurons. The validated protocol and reagents produce highly pure primary neurons for either short- or long-term culture, and the kit is designed for use by both experienced and non-experienced users.
上海嵘崴达实业有限公司,专业经营生命科学领域的科
研试剂、仪器和实验室消耗品,
致力于向国内生命科学研究人员提供合格产品和完善服务。
主要经营的试剂品牌:
Roche试剂,部分常用现货,周三定货,2-3周到货。
GE试剂,耗材,部分常用现货,周二,周五定货,3周左右。
SIGMA试剂,周一,五定货,3周左右到货。
THERMO试剂,部分现货,周三定货,2-3周到货。
ABCAM试剂,国内现货三天左右,期货四周左右。
Peprotech试剂,部分常用现货,周三定货,2-3周到货。
罗氏原料,请来电来邮咨询。
OXOID,BD培养基货期八周左右,部分现货。
MCE激动剂,ACROS、ALFA、阿拉丁等生化试剂现货定购.
| Pierce | 88280 | Pierce Primary Neuron Isolation Kit | DAFA Kit |
| Pierce | 88281 | Pierce Primary Cardiomyocyte Isolation Kit | 1L Kit |
| Pierce | 88282 | Pierce LAL Chromogenic Endotoxin Quantitation Kit | Kit |
| Pierce | 88283 | Neuronal Culture Medium | 500 mL |
| Pierce | 88284 | Hanks’ Balanced Salt Solution (HBSS, without Ca2+, Mg2+) | 500 mL |
| Pierce | 88285 | Neuronal Isolation Enzyme (with papain), lyophilized | 1 VIAL |
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文献和实验序言:国内外关于原代培养有很多文献,不幸的是,没有一篇是详细的,没有一篇解答过why。 为了让大家少走弯路,根据我杀过3000只老鼠的经验,我把我这几年摸索的经验和大家分享,相信你follow我的这篇文章一定会做的很好。 我的标题说的很像吹牛,但是我是严肃认真的说的,I earn it。 note:这篇文章全是我个人的经验,99.9%原创,经过无数失败的到的最完美的原代神经元培养法。 1. 材料选择 一定要严格按照国际上,NCBI数据库,其他文献
相关专题 Thermo Scientific Pierce近日推出了HiPPR去垢剂去除离心柱/离心板,可更快更彻底地从细胞裂解液及其他生物样品中去除去垢剂。这种即用型的产品适用于低浓度的蛋白及多肽样品。HiPPR树脂结合并去除去垢剂,同时很好地回收蛋白及多肽,便于下游的质谱分析。 大家都知道,去垢剂和表面活性剂是制备蛋白和多肽样品时常常使用的成分。然而,它们也会干扰下游的ELISA、等电聚焦和质谱分析。请
Protocol for the Primary Culture of Cortical and Hippocampal neurons Solutions and media required:Poly D-lysine/laminin solution - pdfDM/KY - pdfOptimem - pdfNeuronal growth medium - pdfSet-up for the dissection:Day 1:Add poly D-lysine/laminin
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