Recombinant Human FGFb (146 AA

Recombinant Human FGFb (146 AA)  (C779)

 

产品说明(Description)

Recombinant Human Fibroblast growth factor 2/Fibroblast Growth Factor Basic is produced by our E.coli expression system and the target gene encoding Pro143-Ser288 is expressed. 

Accession #: P09038

Known as: Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of  20mM Tris-Hcl, 250mM NaCl, pH7.5.

 

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:     Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

 

Bioactivity:    Measured in a cell proliferation assay using BALB/c 3T3 cells.

                        The ED50 for this effect is 0.3-2.0 ng/ml.

                        The ED50 for this effect is typically 809.7 pg/mL.（Novoprotein）

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

FGF-basic is a members of the Fibroblast Growth Factors (FGFs) family.The family constitutes a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY