铜－ATP酶测定试剂盒(比色法)

铜－ATP酶测定试剂盒(比色法) 使用注意事项
1． 微量试剂取用前请离心集液。
2． Annexin V-EGFP，Propidium Iodide（PI）避光保存及使用。
3． Propidium Iodide （PI）有毒，操作时请戴手套。
4． 本试剂盒适用于检测活细胞，流式细胞仪检测时，细胞数量不应低于1×105，，不推荐用于检测组织样本。
5． 推荐使用悬浮培养细胞。如果是贴壁细胞，需用不含EDTA的胰酶消化，如消化不当，可能引起假阳性，而用细胞刮子会造成细胞粘连成团，而影响检测。可将胰酶消化后细胞的保存在含2%BSA的PBS中，防止进一步的损伤。
6． 细胞固定后可能导致荧光的淬灭，请不要固定样品。
7． 因检测细胞的类型、凋亡诱导剂种类、使用的检测仪器不同，因而流式检测的荧光补偿也不同，因此建议每次检测均需使用未经凋亡诱导处理的细胞作为对照，进行荧光补偿的调节。
中文名称：铜－ATP酶测定试剂盒(比色法)
英文名称：Copper-ATPase assay kit
产品规格：100管/50样
发货周期：1～3天
检测指标：铜－ATP酶;Cu-ATPase
本试剂盒可测各种动物组织、培养细胞等样本中的Cu2+- ATP酶活性。ATP酶存在于细胞膜及细胞器膜上，在物质运送、能量转换以及信息传递方面具有重要的作用，机体在缺O2及一些疾病状态下，此酶活力发生一系列改变。另外有些遗传疾病也与此酶活力的高低有关。ATP酶可分解ATP生成ADP及无机磷，测定无机磷的量可判断ATP酶活力的高低。Cu2+-ATPase酶是一种能被钾激活而可被钒酸抑制的酶。
ATP酶存在于组织细胞膜及细胞器膜上，是生物膜上的一种蛋白酶，它在物质运送、能量转换以及信息传递方面具有重要的作用，机体在缺O2及一些疾病状态下，此酶活力发生一系列改变，另外有些遗传疾病也与此酶活力的高低有关。
优点如下：
1、快速简便：全程约50分钟，可测100例左右样本。
2、取样量微：本法取样本量100l。
3、稳定性好：试剂盒2～8℃存放6个月有效。
4、再现性好：变异系数CV=1.7%。
5、回收试验： X =96%。
6、受外界影响因素小：干扰因素少，重复性强。
7、测试面广：可测动物组织、红细胞以及各种培养细胞、各种水产等，效果均佳。


Eperythrozoon lenps兔附红细胞体（兔嗜血支原体）PCR试剂盒别    名  C4b binding protein beta chain; C4b-binding protein beta chain; C4BP; C4BPB; C4BPB_HUMAN; Complement component 4 binding protein beta; Complement component 4 binding protein beta chain; Complement component 4-binding protein beta; OTTHUMP00000034288; OTTHUMP00000034289; OTTHUMP00000034375; OTTHUMP0000003474; Proline rich protein; PRP.性    状  Lyophilized or Liquid免 疫 原  KLH conjugated synthetic peptide derived from human C4 binding protein亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon lenps兔附红细胞体（兔嗜血支原体）染料法荧光定量PCR试剂盒别    名  Natural killer cell p44-related protein; CD336; CD336 antigen; LY95; Lymphocyte antigen 95 homolog; Natural cytotoxicity triggering receptor 2; Natural killer cell p44-related protein; NK cell activating receptor; NK p44; NKp44; NCTR2_HUMAN.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Eperythrozoon lenps兔附红细胞体（兔嗜血支原体）探针法荧光定量PCR试剂盒别    名  CC85C_HUMAN; ccdc85c; Coiled coil domain containing 85C; Coiled-coil domain-containing protein 85C; FLJ22558; MGC117455.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:50-200（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Eperythrozoon lirei山羊附红细胞体（山羊嗜血支原体）PCR试剂盒别    名  C10orf46; chromosome 10 open reading frame 46; Cullin; FLJ40409; MGC33215; OTTHUMP00000046870; Uncharacterized protein C10orf46 homolog; CACL1_HUMAN; CDK2-associated and cullin domain-containing protein 1; Cdk-associated cullin1; CACUL1; CAC1.免 疫 原  KLH conjugated synthetic peptide derived from the middle of human CULLIN产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Eperythrozoon lirei山羊附红细胞体（山羊嗜血支原体）染料法荧光定量PCR试剂盒别    名  Androgen receptor N terminal domain transactivating protein 1; Chromosome 20 open reading frame 14; fc12b02; hypothetical protein LOC323855; p102 U5 small nuclear ribonucleoprotein particle binding protein; Pre mRNA processing factor 6; PRP6 homolog; PRPF 6; PRPF6; Putative mitochondrial outer membrane protein import receptor; U5 102 kDa protein; U5 snRNP associated 102 kDa protein; wu:fa05f07; wu:fc12b02; zgc:65913; PRP6_HUMAN.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Eperythrozoon lirei山羊附红细胞体（山羊嗜血支原体）探针法荧光定量PCR试剂盒别    名  Killer cell lectin-like receptor subfamily D member 1; CD 94; CD94 antigen; KLRD 1; KLRD1; KLRD1 protein; KP 43; KP43; Natural killer cells antigen CD94; NK cell receptor.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Eperythrozoon ovis绵羊附红细胞体（绵羊嗜血支原体）PCR试剂盒别    名  ALP; C-C motif chemokine 27; CC chemokine ILC; Ccl27; CTACK; CTAK; Cutaneous T cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESkine; IL 11 R alpha locus chemokine; IL-11 R-alpha-locus chemokine; PESKY; SCYA27; Skinkine; Small inducible cytokine A27; Small-inducible cytokine A27.免 疫 原  KLH conjugated synthetic peptide derived from mouse CCL27 N-terminus产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon ovis绵羊附红细胞体（绵羊嗜血支原体）染料法荧光定量PCR试剂盒别    名  CD_antigen=CD6; CD6; CD6 antigen Tp120; CD6_HUMAN; FLJ44171; OX52; T cell differentiation antigen CD6; T-cell differentiation antigen CD6; T12; TP120.免 疫 原  KLH conjugated synthetic peptide derived from human CD6 C-terminus产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon ovis绵羊附红细胞体（绵羊嗜血支原体）探针法荧光定量PCR试剂盒别    名  Antigen CD48; Antigen CD48; B cell membrane protein; B lymphocyte activation marker BLAST 1 [Precursor]; B lymphocyte activation marker BLAST 1; B-lymphocyte activation marker BLAST-1; BCM 1 surface antigen; BCM1; BCM1 surface antigen; BCM1 surface antigen; BLAST 1; BLAST; BLAST1; BLAST1; CD 48; CD48; CD48 antigen (B cell membrane protein); CD48 antigen; CD48 molecule; CD48 protein; CD48_HUMAN; hCD48; Leucocyte antigen MEM 102; Leukocyte antigen MEM-102; mCD48; MEM 102; MEM102; SLAMF 2; SLAMF2; TCT.1; TCT.1.免 疫 原  KLH conjugated synthetic peptide derived from human CD48产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon perekropovi犬附红细胞体（犬嗜血支原体）PCR试剂盒别    名  C22orf32; Chromosome 22 open reading frame 32; CV032_HUMAN; DDDD; dJ186O1.1; mitochondrial; UPF0466 protein C22orf32.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Eperythrozoon perekropovi犬附红细胞体（犬嗜血支原体）染料法荧光定量PCR试剂盒别    名  Brain protein 16; BRP16; chromosome 8 open reading frame 30A; D15Ertd741e; F203A_HUMAN; Fam203a; FLJ40907; LOC51236; MGC94185; MNCb-5873; Protein FAM203A.免 疫 原  KLH conjugated synthetic peptide derived from human C8orf30A产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon perekropovi犬附红细胞体（犬嗜血支原体）探针法荧光定量PCR试剂盒别    名  Cecropin免 疫 原  KLH conjugated synthetic peptide derived from Hyalophora cecropia产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   ELISA=1:500-1000  IHC-P=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon spp.附红细胞体（嗜血支原体）属通用PCR试剂盒别    名  60S acidic ribosomal protein PO; dJ657E11.4; mRNA turnover 4 homolog; MRT 4; MRT4; MRT4 mRNA turnover 4 homolog; MRTO 4; MRTO4; Ribosomal protein large P0 like; Ribosomal protein P0 like protein; MRT4_HUMAN.免 疫 原  KLH conjugated synthetic peptide derived from human C1orf33产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon spp.附红细胞体（嗜血支原体）属通用染料法荧光定量PCR试剂盒别    名  Ccdc85b; Coiled coil domain containing 85B; Coiled-coil domain-containing protein 85B; Delta interacting protein A; Delta-interacting protein A; DIPA; Hepatitis delta antigen interacting protein A; Hepatitis delta antigen-interacting protein A; CC85B_HUMAN.免 疫 原  KLH conjugated synthetic peptide derived from human CCDC85B产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500亚    型  IgG纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Eperythrozoon spp.附红细胞体（嗜血支原体）属通用探针法荧光定量PCR试剂盒别    名  Claudin-14; CLDN14; DFNB29; Human CLDN14 gene; OTTHUMP00000109046; OTTHUMP00000109049; OTTMUSP00000021531; UNQ777/PRO1571.亚    型  IgG产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500纯化方法  affinity purified by Protein A储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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