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- 详细信息
- 文献和实验
- 技术资料
- 库存:
47
- 英文名:
Nonesterified Free fatty acids assay kit
- 保质期:
详见说明书
- 供应商:
上海一研
- 保存条件:
低温
- 规格:
96T
1. 微量试剂取用前请离心集液。
2. Annexin V-EGFP,Propidium Iodide(PI)避光保存及使用。
3. Propidium Iodide (PI)有毒,操作时请戴手套。
4. 本试剂盒适用于检测活细胞,流式细胞仪检测时,细胞数量不应低于1×105,,不推荐用于检测组织样本。
5. 推荐使用悬浮培养细胞。如果是贴壁细胞,需用不含EDTA的胰酶消化,如消化不当,可能引起假阳性,而用细胞刮子会造成细胞粘连成团,而影响检测。可将胰酶消化后细胞的保存在含2%BSA的PBS中,防止进一步的损伤。
6. 细胞固定后可能导致荧光的淬灭,请不要固定样品。
7. 因检测细胞的类型、凋亡诱导剂种类、使用的检测仪器不同,因而流式检测的荧光补偿也不同,因此建议每次检测均需使用未经凋亡诱导处理的细胞作为对照,进行荧光补偿的调节。
中文名称:游离脂肪酸测试盒(酶法,双试剂)(微板法)
英文名称:Nonesterified Free fatty acids assay kit
产品规格:96T
发货周期:1~3天
检测指标:游离脂肪酸;NEFA
游离脂肪酸能与铜离子结合形成脂肪酸的铜盐而溶于氯仿中,其含量与游离脂肪酸含量成正比。用铜试剂测定其中铜离子的含量,即可推算出游离脂肪酸的含量。
游离脂肪酸,别名非酯化脂肪酸,是脂肪水解产物,测定血清脂肪酸可以了解脂肪代谢的情况,升高代表脂肪分解增加。
Babesia canis犬巴贝斯虫PCR试剂盒别 名 Apoptosis regulator Bax cytoplasmic isoform beta; BAX; Bax B; Bax beta; BaxB; BCL2 associated X protein; BCL2 associated X protein isoform beta.免 疫 原 KLH conjugated synthetic peptide derived from human beta Bax产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia canis犬巴贝斯虫染料法荧光定量PCR试剂盒别 名 Bcl 2 related ovarian killer protein; Bcl-2-like protein 9; Bcl-2-related ovarian killer protein; Bcl2-L-9; BCL2L9; BOK; BOK_HUMAN; BOKL; Hbok; MGC4631.免 疫 原 KLH conjugated synthetic peptide derived from human BOK/Bcl 2 like protein 9 (23-60aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia canis犬巴贝斯虫探针法荧光定量PCR试剂盒别 名 Apoptosis facilitator; Bcl 2 like 13; Bcl 2 like 13 protein; BCL2 like 13 apoptosis facilitator; BCL2 like 13; Bcl2 like 13 protein; BCL2L13; MIL 1; MIL1; MIL1 protein; Protein Mil1; B2L13_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human Bcl rambo/Bcl 2 like 13 protein (291-325aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia divergens分歧巴贝斯虫PCR试剂盒别 名 Apoptosis facilitator Bcl 2 like 14 protein; Apoptosis facilitator Bcl 2 like protein 14; Apoptosis regulator Bcl G; Bcl2 L 14; BCL2 like 14 (apoptosis facilitator); BCL2 like 14; BCL2L14; BCLG; B2L14_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human Bcl G/BCL2 like 14 (265-301aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia divergens分歧巴贝斯虫染料法荧光定量PCR试剂盒别 名 Bcl 2 modifying factor; Bcl-2-modifying factor; Bcl2 modifying factor; Bmf; BMF_HUMAN; FLJ00065.免 疫 原 KLH conjugated synthetic peptide derived from human Bcl2 modifying factor (54-88aa)产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia divergens分歧巴贝斯虫探针法荧光定量PCR试剂盒别 名 BMP 11 BMP11; Bone morphogenetic protein 11; GDF 11; Growth differentiation factor 11; GDF11_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human BMP11产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia equi马巴贝斯虫PCR试剂盒别 名 ZFP36L2; BRF2; Butyrate response factor 2; EGF response factor 2; ERF 2; ERF2; Protein TIS11D; RNF162C; zinc finger protein 36, C3H type like 2.免 疫 原 KLH conjugated synthetic peptide derived from human TIS11D产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia equi马巴贝斯虫染料法荧光定量PCR试剂盒别 名 Bax interacting factor 1; Bax-interacting factor 1; Bif 1; Bif-1; CGI 61; Endophilin B1; Endophilin-B1; SH3 domain GRB2 like endophilin B1; SH3 domain-containing GRB2-like protein B1; Sh3glb1; SH3p13; SHLB1_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human Bif产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia equi马巴贝斯虫探针法荧光定量PCR试剂盒别 名 Bardet Biedl syndrome 12 protein; Bardet-Biedl syndrome 12 (human); Bardet-Biedl syndrome 12 protein homolog; BBS12 gene; C4orf24; FLJ35630; FLJ41559; Gm1805; Gm407; Gm721; RP23-137F6.2;BBS12_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human BBS12产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia gibsoni吉氏巴贝斯虫PCR试剂盒别 名 BTB (POZ) domain containing 3; BTB domain containing 3; BTB/POZ domain containing protein 3; BTB/POZ domain-containing protein 3; BTBD3 protein.免 疫 原 KLH conjugated synthetic peptide derived from human BTBD3产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia gibsoni吉氏巴贝斯虫染料法荧光定量PCR试剂盒别 名 BTB (broad complex tramtrack and bric a brac pox virus and zinc finger) domain containing protein 10 ; BTB (POZ) domain containing 10; BTB domain containing 10; BTB/POZ domain-containing protein 10; GMRP 1; GMRP1; K+ channel tetramerization protein; BTBDA_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human BTBD10产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia gibsoni吉氏巴贝斯虫探针法荧光定量PCR试剂盒别 名 BTB/POZ domain-containing protein 17; btbd17; BTBDH_HUMAN; Galectin-3-binding protein-like.免 疫 原 KLH conjugated synthetic peptide derived from human BTBD17产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia major大巴贝虫PCR试剂盒别 名 BB115071; BTB/POZ domain-containing protein 19; BTBD19; BTBDJ_HUMAN; RP23-59L24.3.免 疫 原 KLH conjugated synthetic peptide derived from human BTBD19产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia major大巴贝虫染料法荧光定量PCR试剂盒别 名 BRIX; Brix domain containing 2; Brix domain containing protein 2; BRIX1; BRX1, biogenesis of ribosomes, homolog (S. cerevisiae); FLJ11100; Ribosome biogenesis protein Brix; BRX1_HUMAN.免 疫 原 KLH conjugated synthetic peptide derived from human BXDC2产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Babesia major大巴贝虫探针法荧光定量PCR试剂盒别 名 BOLA 1; BOLA-1; BolA homolog 1; BolA like 1; BolA like protein 1; BolA-like protein 1; Bola1; BOLA1_HUMAN; CGI 143; hBolA; MGC75015; RP11 196G18.18.免 疫 原 KLH conjugated synthetic peptide derived from human BOLA1产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200亚 型 IgG纯化方法 affinity purified by Protein A储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200(石蜡切片需做抗原修复)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
VSIG4 T淋巴细胞负调节蛋白抗体Rhesus antibody Rh规格 0.1ml
VRK1 牛痘病毒相关激酶1抗体(丝氨酸/苏氨酸蛋白激酶VRK1)Rhesus antibody Rh规格 0.2ml
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VPS18 空泡分选蛋白18抗体Rhesus antibody Rh规格 0.1ml
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游离脂肪酸测试盒(酶法,双试剂)(微板法) 2,4-D 2,4-二苯氧(除草剂)抗体 50次Herba polygoni avicularis萹蓄PCR鉴定试剂盒 负20度 一年2-4周 低温
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5α还原酶检测试剂盒Cynarin进口/国产琥珀美洛尔标准品琥珀美洛尔标准品48T/96T细胞株1x10^6cells
HHV8/ORF K14 人类疱疹病8 ORF14抗体进口/国产猴α干扰素(IFN-α)ELISA Kit,48T/96T
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突状细胞表面特性C型凝集素-细胞间黏附分子3结合非整合素分子检测试剂盒中药对照药材进口/原装普罗布考标准品普罗布考标准品48T/96T//
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plant lectin B4/IB4 植物凝集素IB4进口/分装大鼠α干扰素(IFN-α)ELISA Kit,48T/96T
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脱-γ-羧基凝血酶原检测试剂盒中药对照药材进口/国产哈拉泮标准品哈拉泮标准品48T/96T培养瓶75cm2CRL-1993
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文献和实验酶的酶法反酯化可克服这些缺陷,反应温度很低,甘油回收较简单,且废水处理时不耗费催化剂。另外,废油和脂肪中的游离脂肪酸可完全转化为烷基酯。酶也可回收再用。然而,问题之一是产品成本。另一与酶法反酯化有关的问题是反应速度、产率和产品质量。 São Paulo大学与Maringa大学的研究团队采用不同来源的脂肪酶(Thermomyces lanuginosus; Pseudomonas fluorescens; Burkholderia cepacia; Penicillium
%)。血清PL定量方法包括测定无机磷化学法和酶法两大类。⒈化学测定法包括①抽提分离;②灰化;③显色、比色三个阶段。⒉酶测定法可分别利用磷脂酶A、B、C、D等4种酶作用,加水分解,测定其产物,对PL进行定量,一般多采用磷脂酶D(PL-D)。PL-D可作用于含有卵磷脂、溶血卵磷脂和鞘磷脂以及胆碱的磷脂,这三种磷脂约占血清总磷脂的95%。该法快速准确,便于自动化仪器进行批量检测。 六、游离脂肪酸测定 临床上将C10以上的脂肪酸称为游离脂肪酸(FFA)。正常血清中含有油酸(C18:1)占54%,软脂
hsclgw 用人重组胰岛素来造HepG2细胞的胰岛素抵抗模型好做吗?做完以后仅仅测量用葡萄糖氧化酶法来测量葡萄糖消耗量,就可以判断模型成功了吗? 有没有相关的方法比较全的文献借鉴一下?或者用葡萄糖、游离脂肪酸来诱导胰岛素抵抗的文献哪位大侠有吗? 看了国内外参差不齐的文献,还是有点晕~ 先感谢了!! zhuimenzzhiyn 我目前也在做这方面课题,希望可以交流下,我的
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