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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
att BP clone Enzyme mix
- 保质期:
有效期 2个月
- 供应商:
上海康朗生物科技有限公司
- 保存条件:
长时间储存于 2-8℃避光保存
- 规格:
20T/100ul
产品介绍:
attLR clone Enzyme mix用来催化来自克隆的 PCR 产物或者亚克隆 DNA 片段(含有 attB 位点)与供体载体(含有 attP 位点)进行体外重组,产生入门克隆。 本品包含酶和缓冲剂的单一型混合物,所需的移液步骤更少,因此可以快捷地设置十微升反应体系。
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文献和实验Steady State ATPase Assays Coupled Enzyme System
. 5. Make Tris-MgCl2 -DTT mix for all assays. Tris-MgCl2 -DTT mix = (n = # of assays + 1) n x 20 µL 0.5 M Tris-OAc, pH 7.5 20 µL 10 mM MgCl2 20 µL 10 mM DTT 70 µL DDW
Clone Genes From a Phage Library
by whoever gave it to you. Dilute phage in SM solution to a series of test dilutions. • Mix the phage desired (in about 1 to 50 microliters) with 50 microliters of bacteria and incubate at 30 degrees or 37 degrees for 20minutes. • Place
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
g are used. The most technically difficult of the procedure is the megabase DNA partial digestion and size selection. To date, all large-insert bacterial clone libraries reported were generated from the restriction enzyme partial digests
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