重组大鼠TNF-α现货供应

特别提示：包括重组大鼠TNF-α在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组大鼠TNF-α
英文名称：Recombinant Rat Tumor Necrosis Factor-alpha
产品货号：GS4497
产品规格：5μg

分子量：Approximately 17.2kDa,a single non-glycosylated polypeptide chain containing 156 amino acids.
储存条件：冷冻（-20℃）

概述

Tumor necrosis factor alpha(TNF-α),also called cachectin,is the best-know member of the TNF-family,which can cause cell death.This protein is produced by neutrophils,activated lymphocytes,macrophages,NK cells,LAK cells,astrocytes endothelial cells,smooth muscle cells and some transformed cells.TNF-α occurs as a secreted,soluble form and as a membrane-anchored form,both of which are biologically active.The naturally-occurring form of TNF-α is glycosylated,but non-glycosylated recombinant TNF-α has comparable biological activity.Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞98%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml,corresponding to a specific activity of>2.0×107 IU/mg in the presence of actinomycin D.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.2,150 mM NaCl.
内毒素	Less than 1 EU/μg of rRtTNF-α as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL

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