Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2 (Rat TNF-α/TNFSF2)
规格:
5 μg
Product Information
Product Name
Cat#
Size
Price(¥)
Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2
(Rat TNF-α/TNFSF2)
90628ES08
5 μg
815.00
90628ES60
100 μg
7865.00
90628ES76
500 μg
20425.00
Product Description
Tumor necrosis factor alpha (TNF-α), also known as cachectin, is a cachectin, is a polypeptide cytokine produced by monocytes and macrophages. TNFα receptor signaling can simultaneously activate caspase 8, the transcription factor, NF-κB and the kinase, JNK. Human TNF-α shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. Inflammation is associated with insulin resistance, and TNF-α and IL-6 may affect glucose uptake. A wide variety of evidence has pointed to a critical role of TNF-α in tumour proliferation, migration, invasion and angiogenesis.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 98% by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.