Pierce™ BCA 蛋白检测试剂盒，23225

The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.

Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.

The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.

Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip.  The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.

Features of the BCA Protein Assay Kit include:

Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
Sensitive—detect down to 5 μg/mL with enhanced protocol
No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve