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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Features of the BCA Protein Assay Kit include:
Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
Sensitive—detect down to 5 μg/mL with enhanced protocol
No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve
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文献和实验BCA 蛋白定量法是实验室常用的蛋白质定量方法。Invent 所有蛋白提取试剂盒提取的蛋白均可使用 BCA 法进行定量！那么今天小编就手把手的教大家该如何用 BCA 来定量！BCA 法原理碱性条件下，蛋白将 Cu2+还原为 Cu+，Cu+与 BCA 试剂相互作用形成紫色的络合物，该水溶性的复合物在 562nm 处显示强烈的吸光性，吸光度和蛋白浓度在广泛范围内有良好的线性关系，因此根据吸光值可以推算出蛋白浓度。实验操作具体步骤1. 梯度稀释牛血清白蛋白（BSA）标准品（具体操作按试剂盒中说明
把BCA工作缓冲液和样品滴加在Take3板的微孔处，不需要先在其它容器混匀进行颜色反应。这样做的好处是可以提高操作的便捷性，每次检测只需要2ul样品，非常节约样品。实验材料和方法检测试剂盒（Sigma-Aldrich公司）、小牛血清（BSA）（Sigma-Aldrich公司）。我们使用两种实验方法来进行BCA检测。第一种方法按照NanoDrop技术手册推荐的“Mini-BCA”法。其简要流程是：BCA检测系统混合好的10ulBCA工作缓冲液和10ul的标准蛋白或待测的10μl样品（1:1）在试管中混匀
相关专题 Thermo Scientific Pierce近日推出了HiPPR去垢剂去除离心柱/离心板，可更快更彻底地从细胞裂解液及其他生物样品中去除去垢剂。这种即用型的产品适用于低浓度的蛋白及多肽样品。HiPPR树脂结合并去除去垢剂，同时很好地回收蛋白及多肽，便于下游的质谱分析。 大家都知道，去垢剂和表面活性剂是制备蛋白和多肽样品时常常使用的成分。然而，它们也会干扰下游的ELISA、等电聚焦和质谱分析。请
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