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- MedChemExpress(MCE)
- 美国
- HY-K0221
- 2025年07月14日
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货期：1-2天
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MedChemExpress LLC
- 规格：
1 mL/3 mL/6 mL/12 mL/30 mL/60 mL
| 规格： | 1 mL | 产品价格： | ￥240.0 |
|---|---|---|---|
| 规格： | 3 mL | 产品价格： | ￥240.0 |
| 规格： | 6 mL | 产品价格： | ￥280.0 |
| 规格： | 12 mL | 产品价格： | ￥320.0 |
| 规格： | 30 mL | 产品价格： | ￥320.0 |
| 规格： | 60 mL | 产品价格： | ￥380.0 |
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亲和层析柱
Affinity Chromatography Column 可以搭配填料，用于纯化具有不同标签的酶、抗体、抗原、重组蛋白等。
Description & Advantages:
MCE Affinity Chromatography Column is made of high-purity polypropylene with a very low adsorption to various biomolecules. Frits for AC column are made of UHWMPE, which is hydrophilic, and optimized for minimizing non-specific adsorption.
• Minimizing non-specific adsorption.
• Excellent hydrophilic performance.
• Can be used for substances of low concentration.
• Rapid separation is achieved while avoiding contamination.
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文献和实验Affinity Column Preparation 亲和层析柱制备
and rotate in the 15 ml tube for at least 5 hours at room temperature.7.Wash away lysine by 4 washes of 10 ml each of PBS.8.Add PBS to the beads and pour them into a small plastic column.Run PBS through the column to pack the beads,and store the column
Affinity Column Preparation--亲和层析柱制备
Affinity Column Preparation 亲和层析柱制备 Peter Novick Lab,Department of Cell Biology Yale University School of Medicine1.Prepare 1 ml of packed beads by washing them 4 times in 10 ml of d-H2O in a 15 ml tube.The beads are Act.Ultrogel 22 AcA from IBF
Preparation of Affinity Column制备亲和层析柱【UCSF】
and vacuum.Do not allow beads to dry. 5.Wash bed with 3x2ml dH2O. 6.Scrape 0.5-1ml into 15ml microfuge tube. 7.Add 2.22ml CREBtide ligand solution to tube. Rock at 4℃ for 4hrs. 8.Transfer slurry to column. 9.Wash 2x500μl dH2O. 10.Collect eluate
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