MCE 2× PCR Master Mix (with Dye) is a convenient, ready-to-use kit for PCR. The 2× PCR Master Mix contains all the reagents necessary for routine PCR, including Taq DNA Polymerase, dNTPs Mix, MgCl2 and optimized reaction buffer. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. After the PCR reaction, the PCR products can be loaded directly onto an agarose gel. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. The obtained PCR products contain poly(A) at the 3'-end and can be directly cloned into T-Vectors.