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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海谷研
- 库存:
37
- 规格:
10ug
产品名称:Recombinant Mouse CXCL15/Lungkine
Known as C-X-C motif chemokine 15;Cxcl15;Lungkine;Small-inducible cytokine B15;Scyb15
Derived from Human cells
Purity Greater than 95% as determined by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.
Shipping The product is shipped at ambient temperature.
Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Endotoxin Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Background Mouse C-X-C motif chemokine 15, also known as Cxcl15, is a secreted protein which is member of the ELR motif-containing CXC chemokines. It expressed at low levels in fetal lung, the expression restricted to the lung, produced by bronchoepithelial cells and is released into the airways. It plays an important role in lung-specific neutrophil trafficking during normal and inflammatory conditions. It also appears chemotactic for neutrophils.
Recombinant Mouse C-X-C motif chemokine 15 is produced by our Mammalian expression system and the target gene encoding Gln26-Ala167 is expressed with a 6His tag at the C-terminus.
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文献和实验with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
PRIMARY MOUSE KERATINOCYTE CULTURES Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol . 254:3-20 (1995). The epidermis from a newborn mouse should yield
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