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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
充足
- 供应商:
ExCell Bio
- 英文名:
Fetal Bovine Serum( Prime)
- 规格:
500ml
胎牛血清是指胎牛血液凝固后,去除血浆中纤维蛋白原及某些凝血因子后分离出的淡黄色澄清液体,它是肉类加工业的一个副产品。
胎牛血清是细胞培养中用量较大的天然培养基,含有丰富的细胞生长必须的营养成分,具有极为重要的功能,一般添加比例为:5-20%。血清中含有各种氨基酸、维生素、无机物、脂类物质等这些维持细胞生长必须的营养物质,也含有胰岛素、氢化可的松、地塞米松、bFGF、EGF、PDGF、转铁蛋白等可促进细胞生长的激素、生长因子及结合蛋白。此外,血清还有解毒、缓冲、抑制蛋白酶活性等作用保护细胞不受伤害。
依科赛胎牛血清均采自非疫区的健康牛,经无菌采集、批量混合、3级100 nm过滤器过滤分装而成,不含支原体和BVDV、PI3、IBR、BTV等牛源病毒。FSP胎牛血清是一款适用于常规细胞系、传代细胞系及部分原代细胞的培养的优级胎牛血清,适用细胞类型:
- 293、293T、CHO、Vero、SF-9等传代细胞;
- Hela、A549、HepG2、MCF-7、HeDG2等肿瘤细胞;
- MRC-5、KMB-17、2BS等二倍体细胞
- 乳腺癌细胞(MDA-MB-231)、鼻咽癌细胞(NPC;SUNE1;CNE1;CNE2;HNE1;HONE1;HNE2;TW03)、结肠癌(CT-26;HCT116;HT-29;HCT8;LoVo;MC38)、胰腺癌(Panco2)、膀胱癌( T24)、肝癌细胞(Huh7)等肿瘤细胞;
- B细胞、SP2/0等骨髓瘤细胞、杂交瘤细胞;
FSP胎牛血清血源源自非疫区的南美,并在南美加工生产,产品经3级100nm过滤, 符合国际知名胎牛血清检测标准。适用于部分原代细胞及其他细胞的培养。原装进口、高质低价。
1.内毒素含量≤10EU/ml
2.血红蛋白含量≤0.02%(w/v)
3.促细胞生产曲线峰值>1.2×106/ml
4.细胞倍增时间<16小时
5.细胞克隆率>70%
6.BVD 、PI3 、IBR检测均为阴性(未检出)



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文献和实验Zhong B et al. The Ubiquitin Ligase RNF5Regulates Antiviral Responses by Mediating Degradation of the Adaptor ProteinMITA. Volume 30, ISSUE 3, P397-407, March 20, 2009. Read more(PubMed: 19285439)
Li Q et al. CRISPR–Cas9-mediatedbase-editing screening in mice identifies DND1 amino acids that are criticalfor primordial germ cell development. Nature cell biology,2018. Read more(PubMed: 30718859)
Luo YL etal. Macrophage-Specific in Vivo Gene Editing Using Cationic Lipid-AssistedPolymeric Nanoparticles. ACS Nano 2018, 12, 994−1005.Read more(PubMed:29314827)
Du JZ et al. Smart Superstructureswith Ultrahigh pHSensitivity for Targeting Acidic Tumor. ACS Nano 2016, 10,6753−6761.Read more(PubMed:27244096)
Chen X et al. Long noncoding RNA LBCS inhibits self-renewal andchemoresistance of bladder cancer stem cells through epigenetic silencing ofSOX2. Clinical Cancer Research ,2018. Read more(PubMed:30397178)
Li HJ et al. Stimuli-responsiveclustered nanoparticles for improved tumor penetration and therapeuticefficacy. PNAS,2018. Read more(PubMed:27035960)
Li Y et al. ISG56 is anegative-feedback regulator of virus-triggered signaling and cellular antiviral response. PNAS, 2009,106(19): 7945-7950.Read more(PubMed:19416887)
Wang JL et al. The effect ofsurface poly(ethylene glycol) length on in vivo drug delivery behaviors ofpolymeric nanoparticles. Biomaterials 182(2018)104-113.Read more(PubMed:30114562)
Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Culture of BEND Cells (Bovine Endometrial Cells)
Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse serum (H1138) Antibiotic-antimycotic (ABAM) (A9909) D-valine (V1255) Insulin (I5500) Preparation 1. Place 1000 ml of double distilled water
Primary Cultures of Astrocytes from Fetal Bovine Brain
differentiation and the prevalence of glial cells vs. neurons. We compare explants from early, middle, and late stages of development and two different fetal calf serum concentrations (1 and 10%) to identify the best conditions to obtain
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