- 询价
- Cell Signaling Technology
- 5134
- USA
- 2025年07月15日
- W, IF-IC
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
RCC1 (D15H6) Rabbit mAb
- 抗原：
synthetic peptide corresponding to central residues of human RCC1 protein
- 应用范围：
W, IF-IC
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Mk
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 45 | Rabbit IgG |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | RCC1 (D15H6) Rabbit mAb detects endogenous levels of total RCC1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RCC1 protein. Western Blotting
Western blot analysis of extracts from various cell types using RCC1 (D15H6) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells using RCC1 (D15H6) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). |
| Background | The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 (RCC1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity (6).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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