Phospho-SMC1 (Ser360) Antibody

4029:

Immunofluorescence , Western Blotting

Phospho-SMC1 (Ser360) Antibody detects endogenous levels of SMC1 protein only when phosphorylated on Ser360. This antibody does not cross-react with other SMC proteins.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser360 of the human SMC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or UV-treated (100 J/m² followed by 4 hour recovery) using Phospho-SMC1 (Ser360) Antibody (upper) or SMC1 Antibody #4802 (lower). Antibody phospho-specificity was determined by treating cell extracts with λ phosphatase.

IF-IC

Confocal immunfluorescent analysis of HeLa cells, untreated (left) and UV-treated (right), using Phospho-SMC1 (Ser360) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).

The serine residue at 360 of SMC1 is phosphorylated in an ATM/ATR-dependent manner in response to DNA damage (5,6). Phospho-SMC1 (Ser360) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan^® , CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser360 was discovered using an ATM/ATR substrate antibody and was shown to be induced by UV treatment. Please visit PhosphoSitePlus^® , CST's modification site knowledgebase, at www.phosphosite.org for more information.

1. Michaelis, C. et al. (1997) Cell 91, 35-45.
2. Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5.
3. Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82.
4. Kim, S.T. et al. (2002) Genes Dev 16, 560-70.
5. Stokes, M.P. et al. (2007) Proc Natl Acad Sci U S A 104, 19855-60.
6. Matsuoka, S. et al. (2007) Science 316, 1160-6.

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• 4805 Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb
• 4802 SMC1 Antibody
• 4526 Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb
• 2873 ATM (D2E2) Rabbit mAb
• 2853 Phospho-ATR (Ser428) Antibody
• 2790 ATR Antibody
• 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
• 2909 Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.