- 询价
- Cell Signaling Technology
- 2281
- USA
- 2025年09月03日
- W, IF-F
- Rabbit
- H,M,R,Mk
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- 详细信息
- 技术资料
- 抗体英文名：
SPAK Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ser436 of human SPAK
- 应用范围：
W, IF-F
- 宿主：
Rabbit
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F | H M R Mk | Endogenous | 65 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | SPAK Antibody detects endogenous levels of total SPAK protein. This antibody does not cross-react with OSR1 or other members of the GCK family. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser436 of human SPAK. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from 293, C2C12 and NBT-II cells, using SPAK Antibody. IF-F
Confocal immunofluorescent analysis of choroid plexus epithelial cells showing apical membrane localization, using SPAK Antibody (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3, 4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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