Applications Key: W=Western Blotting IP=Immunoprecipitation Reactivity Key: H=Human Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Cdx2 Antibody detects endogenous levels of total Cdx2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the N-terminus of human Cdx2. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from DLD-1 and MCF7 (Cdx2-null) cells using Cdx2 Antibody.
Background
Cdx2, a homeobox domain-containing transcription factor, is a master regulator of the trophoectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). Cdx2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies such as Barret Esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in Cdx2 display limited hematopoietic progenitor development and altered Hox gene expression (6), pointing to a role for Cdx2 in Hox gene regulation. Cdx2 is also implicated in the aberrant expression of Hox genes in human AML cell lines (7).