- 询价
- Cell Signaling Technology
- 5727
- USA
- 2025年11月17日
- W, IP
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
MEKK3 (D36G5) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Pro310 of human MEKK3 protein
- 应用范围：
W, IP
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 78 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | MEKK3 (D36G5) Rabbit mAb recognizes endogenous levels of total MEKK3 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro310 of human MEKK3 protein. Western Blotting
Western blot analysis of extracts from various cell types using MEKK3 (D36G5) Rabbit mAb. |
| Background | MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2 (1,2). MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins (3,4). MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA (5-9). Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation (10-12), at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ/NF-κB activation (13). Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity (12). Phosphorylation of MEKK3 at Thr294 is diminished upon treatment of cells with LPS or TNFα, further suggesting an inhibitory role for this site (12).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane was incubated
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
Methods for the Detection of D-Amino-Acid Oxidase
min then twice for 5 min. It was incubated for 1 hr in TBS-T containing rabbit anti-hog D-amino-acid oxidase IgG (1/3,000 dilution) and quickly rinsed twice with TBS-T and further washed in TBS-T once for 15 min then twice for 5 min. The membrane
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