- 询价
- Cell Signaling Technology
- 4436
- USA
- 2025年12月25日
- W
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CaMKII (pan) (D11A10) Rabbit mAb
- 抗原：
synthetic peptide surrounding Val184 of human CaMKII-alpha
- 应用范围：
W
- 供应商：
CST
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R | Endogenous | 60, 50 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | CaMKII (pan) (D11A10) Rabbit mAb detects endogenous levels of total CaMKII protein. The peptide sequence used as the antigen is 100% conserved between CaMKII-alpha, gamma and delta, and 88% conserved in CaMKII-beta. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Val184 of human CaMKII-alpha. Western Blotting
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or expressing CaMKII-α (lane2), CaMKII-β (lane 3) or CaMKII-γ (lane 4) using CaMKII (pan) (D11A10) Rabbit mAb. Western Blotting
Western blot analysis of extracts from mouse and rat brain using CaMKII (pan) (D11A10) Rabbit mAb. |
| Background | CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+ /calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
ELISPOT (Enzyme-linked ImmunoSPOT)
Mouse Cytokine ELISPOT Set Catalog No. Capture mAb Detection mAb IFN-γ 88-7384 AN-18 R4-6A2 IL-1α 88-7813 ALF-161 Rabbit
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